Blind Deconvolution of Anisoplanatic Aberrations
A computational correction of microscopic images
wouter van de Ketterij (TU Delft - Mechanical Engineering)
Michel Verhaegen – Mentor (TU Delft - Team Raf Van de Plas)
Oleg Soloviev – Graduation committee member (TU Delft - Team Raf Van de Plas)
Erik Steur – Graduation committee member (TU Delft - Team Bart De Schutter)
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Abstract
Correction techniques, such as tangential iterative projections (TIP), were developed to reconstruct the image for the whole field of view. However, due to the three dimensional nature of biological tissues, induced aberrations are different throughout the field of view. This Master of Science thesis shows the development of four algorithms. These algorithms apply TIP to deconvolve images locally. Thereafter, the local results are combined in order to restore images from anisoplanatic aberrations. The difference between the the algorithms developed during this research is the complexity in the spatial domain and in the Fourier domain. It was found that adaptive limited support in the spatial domain increases the im- age quality of the estimated object. A novel approach for multi-frame deconvolution in the Fourier domain has shown to be a promising modification. The use of weighted multi-frame deconvolution in the Fourier domain has lead to improved image quality.