An Efficient Strategy for the Production of Epoxidized Oils

Natural Deep Eutectic Solvent-Based Enzymatic Epoxidation

Journal article (2019)

Authors

Tianyu Zhang South China University of Technology
Yunjian Ma South China University of Technology
Chin Ping Tan Universiti Putra Malaysia
F. Hollmann BT/Biocatalysis - AS
Jianrong Wang South China University of Technology
Bo Yang South China University of Technology
Yonghua Wang South China University of Technology
Research Group
BT/Biocatalysis (AS) (TU Delft)
Copyright: © 2019 Tianyu Zhang, Yunjian Ma, Chin Ping Tan, F. Hollmann, Jianrong Wang, Bo Yang, Yonghua Wang
DOI: https://doi.org/10.1002/aocs.12220
Lipase Epoxidation Enzyme catalysis Natural deep eutectic solvent Soybean oil
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Published Date

2019

Language

English

Reuse Rights

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Faculty

Applied Sciences

Department

BT/Biotechnologie

Research Group

BT/Biocatalysis

Abstract


Poor H
2
O
2
-resistance by enzymes is a key bottleneck in the epoxidation process of oil by enzymatic methods. In this study, the stability of three lipases, from Aspergillus oryzae lipase (AOL), Aspergillus fumigatus lipase B (AflB), and marine Janibacter (MAJ1), in the presence of H
2
O
2
was evaluated in different types of natural deep eutectic solvents (NADES). This stability was strengthened significantly in the NADES compared to the buffer. Specifically, AOL retained 84.7% of its initial activity in the presence of choline chloride/sorbitol (1:1 M ratio) and 3 mol L
−1
H
2
O
2
after 24 h incubation at 40°C. In addition, the two-phase epoxidation process was optimized with AOL in ChCl/sorbitol to reach up to 96.8% conversion under the optimized conditions (molar ratio of octanoic acid/H
2
O
2
/C=C-bonds = 0.3:1.5:1, enzyme loading of 15 U g
−1
of soybean oil, ChCl/sorbitol content of 70.0% of the weight of hydrophilic phase, and reaction temperature of 50°C). Moreover, the lipase dispersed in NADES retained approximately 66% of its initial activity after being used for seven batch cycles. Overall, NADES-based enzymatic epoxidation is a feasible and promising strategy for the synthesis of epoxidized oils.