The effect of scaffolds with a negative, zero, or positive Poisson’s ratio on pre-osteoblastic (MC3T3-E1) cell response

Master thesis (2020)

Authors

D. Peeters Mechanical Engineering

Contributors

H.M.A. Kolken Biomaterials & Tissue Biomechanics - Mechanical, Maritime and Materials Engineering (coach)
Mohammad J. Mirzaali Biomaterials & Tissue Biomechanics - Mechanical, Maritime and Materials Engineering (mentor)
E.L. Fratila-Apachitei Biomaterials & Tissue Biomechanics - Mechanical, Maritime and Materials Engineering (graduation committee member)
Faculty
Mechanical Engineering, Mechanical Engineering
Copyright: © 2020 Denise Peeters
More Info
expand_more

Published Date

30-11-2020

Language

English

Reuse Rights

Other than for strictly personal use, it is not permitted to download, forward or distribute the text or part of it, without the consent of the author(s) and/or copyright holder(s), unless the work is under an open content license such as Creative Commons.

Faculty

Mechanical Engineering

Abstract

Bone tissue engineering (BTE) researches the characteristics which are needed to create the ultimate bone scaffold which enhances cell response. Limited research has been done regarding the effect of the Poisson’s ratio on the scaffold-cell interaction. In this graduation project we therefore explore the cell response on scaffolds with a different value of the Poisson’s ratio. Various meta-biomaterials were designed, manufactured, mechanically tested and the response on pre-osteoblasts (MC3T3-E1) was explored. The first experiment was performed at mesoscale. The meta-biomaterials, with cells, were evaluated with SEM imaging, presto blue and ARS staining. The second experiment was performed at micro-scale. The results of this experiment were evaluated with SEM imaging, actin staining and Runx2 staining. It was concluded that the auxetic meta-biomaterial, with negative Poisson’s ratio, high porosity and high stiffness, showed an enhancement of the cell response. However, this could not be confirmed by the 2D SEM images. A potential application for the meta-biomaterial that enhances the cell response is implementing this meta-biomaterial in a design for the surface of an implant to generate fast bone ingrowth.

Files

Thesis_Denise_Peeters_Final.pd... (.pdf)
(.pdf | 8.04 Mb)
- Embargo expired in 30-11-2022