Proteomic analysis of methanonatronarchaeum thermophilum AMET1, a representative of a putative new class of euryarchaeota, “methanonatronarchaeia”
Manuel Ferrer (University of the Balearic Islands)
Dimitry Y. Sorokin (Russian Academy of Sciences, TU Delft - BT/Environmental Biotechnology)
Yuri I. Wolf (National Library of Medicine)
Sergio Ciordia (University of the Balearic Islands)
María Carmen Mena (University of the Balearic Islands)
Rafael Bargiela (University of the Balearic Islands)
Eugene V. Koonin (National Library of Medicine)
Kira S. Makarova (National Library of Medicine)
More Info
expand_more
Other than for strictly personal use, it is not permitted to download, forward or distribute the text or part of it, without the consent of the author(s) and/or copyright holder(s), unless the work is under an open content license such as Creative Commons.
Abstract
The recently discovered Methanonatronarchaeia are extremely halophilic and moderately thermophilic methyl-reducing methanogens representing a novel class-level lineage in the phylum Euryarchaeota related to the class Halobacteria. Here we present a detailed analysis of 1D-nano liquid chromatography–electrospray ionization tandem mass spectrometry data obtained for “Methanonatronarchaeum thermophilum” AMET1 grown in different physiological conditions, including variation of the growth temperature and substrates. Analysis of these data allows us to refine the current understanding of the key biosynthetic pathways of this triple extremophilic methanogenic euryarchaeon and identify proteins that are likely to be involved in its response to growth condition changes.
help_outline