Preparation of D-xylulose from D-xylose

Journal article (1988)

Authors

J.T. Pronk BT/Biotechnologie

A.W. Bakker

H.E. van Dam

Adrie J.J. Straathof

W.A. Scheffers

J.P. van Dijken

Department

BT/Biotechnologie

Xylose isomerase Immobilization Acinetobacter calcoaceticus D-xylulose

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Published Date

1988

Language

English

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Department

BT/Biotechnologie

Abstract

A simple method is described for the preparation of d-xylulose. It consists of the isomerization of d-xylose with xylose isomerase (EC 5.3.1.5), yielding an equilibrium mixture of d-xylulose and d-xylose. This is followed by the quantitative oxidation of residual d-xylose to d-xylonic acid with immobilized A. calcoaceticus cells. A combination of methanol precipitation and ion exchange is used for the removal of xylonic acid. This procedure offers many advantages over existing methods for the preparation of d-xylulose. The purity of the final product compares favorably to that of a commercial d-xylulose preparation.