In vivo, in situ and ex vivo comparison of porcine skin for microprojection array penetration depth, delivery efficiency and elastic modulus assessment

In vivo, in situ and ex vivo comparison of porcine skin for microprojection array penetration depth, delivery efficiency and elastic modulus assessment

Wei, J.C. (TU Delft Medical Instruments & Bio-Inspired Technology; University of Queensland)
Cartmill, Ian D. (University of Queensland)
Kendall, Mark AF (University of Queensland; Australian National University)
Crichton, Michael L. (University of Queensland; Heriot-Watt University)

2022

With the development of wearable technologies, the interfacial properties of skin and devices have become much more important. For research and development purposes, porcine skin is often used to evaluate device performance, but the differences between in vivo, in situ and ex vivo porcine skin mechanical properties can potentially misdirect investigators during the development of their technology. In this study, we investigated the significant changes to mechanical properties with and without perfusion (in vivo versus in vitro tissue). The device focus for this study was a skin-targeting Nanopatch vaccine microneedle device, employed to assess the variance to key skin engagement parameters – penetration depth and delivery efficiency – due to different tissue conditions. The patches were coated with fluorescent or ¹⁴C radiolabelled formulations for penetration depth and delivery efficiency quantification in vivo, and at time points up to 4 h post mortem. An immediate cessation of blood circulation saw mean microneedle penetration depth fell from ∼100 μm to ∼55 μm (∼45%). Stiffening of underlying tissues as a result of rigor mortis then augmented the penetration depths at the 4 h timepoint back to ∼100 μm, insignificantly different (p = 0.0595) when compared with in vivo. The highest delivery efficiency of formulation into the skin (dose measured in the skin excluding leftover dose on skin and patch surfaces) was also observed at this time point of ∼25%, up from ∼2% in vivo. Data obtained herein progresses medical device development, highlighting the need to consider the state and muscle tissues when evaluating prototypes on cadavers.

Elastic modulus
Histology
Medical device
Microneedles
Skin
Soft tissue biomechanics

http://resolver.tudelft.nl/uuid:064105ce-664a-468b-aea4-1ff31ec85c61

https://doi.org/10.1016/j.jmbbm.2022.105187

1751-6161

Journal of the Mechanical Behavior of Biomedical Materials, 130

Institutional Repository

journal article

© 2022 J.C. Wei, Ian D. Cartmill, Mark AF Kendall, Michael L. Crichton