Print Email Facebook Twitter Synthetic Polymers Provide a Robust Substrate for Functional Neuron Culture Title Synthetic Polymers Provide a Robust Substrate for Functional Neuron Culture Author Zhang, Yichuan (The University of Edinburgh; Chinese Academy of Sciences) Venkateswaran, Seshasailam (The University of Edinburgh) Higuera, Gustavo A. (Erasmus MC) Nath, Suvra (Student TU Delft) Shpak, Guy (Erasmus MC) Matray, Jeffrey (Student TU Delft) Fratila-Apachitei, E.L. (TU Delft Biomaterials & Tissue Biomechanics) Zadpoor, A.A. (TU Delft Biomaterials & Tissue Biomechanics) Kushner, Steven A. Bradley, Mark (The University of Edinburgh) De Zeeuw, Chris I. (Erasmus MC) Date 2020 Abstract Substrates for neuron culture and implantation are required to be both biocompatible and display surface compositions that support cell attachment, growth, differentiation, and neural activity. Laminin, a naturally occurring extracellular matrix protein is the most widely used substrate for neuron culture and fulfills some of these requirements, however, it is expensive, unstable (compared to synthetic materials), and prone to batch-to-batch variation. This study uses a high-throughput polymer screening approach to identify synthetic polymers that supports the in vitro culture of primary mouse cerebellar neurons. This allows the identification of materials that enable primary cell attachment with high viability even under “serum-free” conditions, with materials that support both primary cells and neural progenitor cell attachment with high levels of neuronal biomarker expression, while promoting progenitor cell maturation to neurons. Subject central nervous system regenerationneuron culturespolymer microarraysprogenitor cell maturationsynthetic polymer substrates To reference this document use: http://resolver.tudelft.nl/uuid:0711096c-c47e-464b-a59a-8282bb306423 DOI https://doi.org/10.1002/adhm.201901347 ISSN 2192-2640 Source Advanced Healthcare Materials, 9 (4) Part of collection Institutional Repository Document type journal article Rights © 2020 Yichuan Zhang, Seshasailam Venkateswaran, Gustavo A. Higuera, Suvra Nath, Guy Shpak, Jeffrey Matray, E.L. Fratila-Apachitei, A.A. Zadpoor, Steven A. Kushner, Mark Bradley, Chris I. De Zeeuw Files PDF Zhang_et_al_2020_Advanced ... erials.pdf 1.92 MB Close viewer /islandora/object/uuid:0711096c-c47e-464b-a59a-8282bb306423/datastream/OBJ/view