Print Email Facebook Twitter Voltage Imaging with Engineered Proton-Pumping Rhodopsins Title Voltage Imaging with Engineered Proton-Pumping Rhodopsins: Insights from the Proton Transfer Pathway Author Meng, X. (TU Delft ImPhys/Brinks group) Ganapathy, S. (TU Delft ImPhys/Brinks group; University of California) van Roemburg, Lars (Student TU Delft) Post, M.G. (TU Delft ImPhys/Brinks group) Brinks, D. (TU Delft ImPhys/Brinks group; Erasmus MC) Date 2023 Abstract Voltage imaging using genetically encoded voltage indicators (GEVIs) has taken the field of neuroscience by storm in the past decade. Its ability to create subcellular and network level readouts of electrical dynamics depends critically on the kinetics of the response to voltage of the indicator used. Engineered microbial rhodopsins form a GEVI subclass known for their high voltage sensitivity and fast response kinetics. Here we review the essential aspects of microbial rhodopsin photocycles that are critical to understanding the mechanisms of voltage sensitivity in these proteins and link them to insights from efforts to create faster, brighter and more sensitive microbial rhodopsin-based GEVIs. Subject biosensorsfluorescence microscopygenetically encoded voltage indicatorsmicrobial rhodopsinsneurosciencephotophysicsprotein engineeringproton transfer pathway To reference this document use: http://resolver.tudelft.nl/uuid:0d808650-4e96-4a01-b69d-652b26ad7bfb DOI https://doi.org/10.1021/acsphyschemau.3c00003 Source ACS Physical Chemistry Au, 3 (4), 320-333 Part of collection Institutional Repository Document type review Rights © 2023 X. Meng, S. Ganapathy, Lars van Roemburg, M.G. Post, D. Brinks Files PDF acsphyschemau.3c00003.pdf 9.37 MB Close viewer /islandora/object/uuid:0d808650-4e96-4a01-b69d-652b26ad7bfb/datastream/OBJ/view