Print Email Facebook Twitter Demystifying polyphosphate-accumulating organisms relevant to wastewater treatment Title Demystifying polyphosphate-accumulating organisms relevant to wastewater treatment: A review of their phylogeny, metabolism, and detection Author Ruiz Hadad, L. (King Abdullah University of Science and Technology) Ali, Muhammad (Trinity College Dublin) Pronk, M. (TU Delft BT/Environmental Biotechnology) van Loosdrecht, Mark C.M. (TU Delft BT/Environmental Biotechnology) Saikaly, Pascal E. (King Abdullah University of Science and Technology) Date 2024 Abstract Currently, the most cost-effective and efficient method for phosphorus (P) removal from wastewater is enhanced biological P removal (EPBR) via polyphosphate-accumulating organisms (PAOs). This study integrates a literature review with genomic analysis to uncover the phylogenetic and metabolic diversity of the relevant PAOs for wastewater treatment. The findings highlight significant differences in the metabolic capabilities of PAOs relevant to wastewater treatment. Notably, Candidatus Dechloromonas and Candidatus Accumulibacter can synthesize polyhydroxyalkanoates, possess specific enzymes for ATP production from polyphosphate, and have electrochemical transporters for acetate and C4-dicarboxylates. In contrast, Tetrasphaera, Candidatus Phosphoribacter, Knoellia, and Phycicoccus possess PolyP-glucokinase and electrochemical transporters for sugars/amino acids. Additionally, this review explores various detection methods for polyphosphate and PAOs in activated sludge wastewater treatment plants. Notably, FISH-Raman spectroscopy emerges as one of the most advanced detection techniques. Overall, this review provides critical insights into PAO research, underscoring the need for enhanced strategies in biological phosphorus removal. Subject Ca. accumulibacterCa. phosphoribacterEnhanced biological phosphorus removal (EBPR)KnoelliaPhycicoccusPolyphosphate-accumulating organisms (PAOs)Tetrasphaera To reference this document use: http://resolver.tudelft.nl/uuid:0eca0fc2-807b-45e8-8fb6-046e9163a8c1 DOI https://doi.org/10.1016/j.ese.2024.100387 Source Environmental Science and Ecotechnology, 21 Part of collection Institutional Repository Document type review Rights © 2024 L. Ruiz Hadad, Muhammad Ali, M. Pronk, Mark C.M. van Loosdrecht, Pascal E. Saikaly Files PDF 1-s2.0-S2666498424000012-main.pdf 3.38 MB Close viewer /islandora/object/uuid:0eca0fc2-807b-45e8-8fb6-046e9163a8c1/datastream/OBJ/view