Print Email Facebook Twitter Looping the Genome with SMC Complexes Title Looping the Genome with SMC Complexes Author Kim, Eugene (Max Planck Insitute of Biophysics, Frankfurt) Barth, R. (TU Delft BN/Cees Dekker Lab) Dekker, C. (TU Delft BN/Cees Dekker Lab) Date 2023 Abstract SMC (structural maintenance of chromosomes) protein complexes are an evolutionarily conserved family of motor proteins that hold sister chromatids together and fold genomes throughout the cell cycle by DNA loop extrusion. These complexes play a key role in a variety of functions in the packaging and regulation of chromosomes, and they have been intensely studied in recent years. Despite their importance, the detailed molecular mechanism for DNA loop extrusion by SMC complexes remains unresolved. Here, we describe the roles of SMCs in chromosome biology and particularly review in vitro single-molecule studies that have recently advanced our understanding of SMC proteins. We describe the mechanistic biophysical aspects of loop extrusion that govern genome organization and its consequences. Subject DNA loop extrusiongenome organizationsingle-molecule studiesSMC complexes To reference this document use: http://resolver.tudelft.nl/uuid:1ba0d52e-a233-4d54-b8ec-4700ecee5997 DOI https://doi.org/10.1146/annurev-biochem-032620-110506 Source Annual Review of Biochemistry, 92, 15-41 Part of collection Institutional Repository Document type review Rights © 2023 Eugene Kim, R. Barth, C. Dekker Files PDF annurev_biochem_032620_110506.pdf 3.27 MB Close viewer /islandora/object/uuid:1ba0d52e-a233-4d54-b8ec-4700ecee5997/datastream/OBJ/view