Print Email Facebook Twitter Unraveling energy homeostasis in a dynamic model of glycolysis in Escherichia coli Title Unraveling energy homeostasis in a dynamic model of glycolysis in Escherichia coli Author Giordano, G. (TU Delft Team Tamas Keviczky) de Graaf, Lotte (Student TU Delft) Vasilakou, E. (TU Delft OLD BT/Cell Systems Engineering) Wahl, S.A. (TU Delft OLD BT/Cell Systems Engineering) Date 2019 Abstract We study dynamic metabolic models that describe the cellular responses of microorganisms under changing environmental conditions, such as substrate perturbations. Feast-famine experiments in Escherichia coli show that, despite the enormous extracellular perturbations, important quantities such as the cell energy charge remain practically constant. We propose a simplified kinetic model of glycolysis in E. coli and we analyse it to investigate the mechanisms that guarantee the observed homeostatic energy charge. Identifying the source of this extraordinary robustness will streamline the synthesis of robust and efficient “cell factories” for the production of relevant chemicals, enabling sustainable processes. To reference this document use: http://resolver.tudelft.nl/uuid:31274967-d586-404c-b672-ae61d6af7312 DOI https://doi.org/10.23919/ECC.2019.8795884 Publisher IEEE, Pisacataway, NJ, USA ISBN 978-3-907144-00-8 Source Proceedings of the 18th European Control Conference (ECC 2019) Event ECC 2019: 18th European Control Conference, 2019-06-25 → 2019-06-28, Napoli, Italy Bibliographical note Accepted Author Manuscript Part of collection Institutional Repository Document type conference paper Rights © 2019 G. Giordano, Lotte de Graaf, E. Vasilakou, S.A. Wahl Files PDF GGVW_ECC2019.pdf 571.3 KB Close viewer /islandora/object/uuid:31274967-d586-404c-b672-ae61d6af7312/datastream/OBJ/view