Print Email Facebook Twitter Simultaneous nitrification and phosphate removal by bioaugmented aerobic granules treating a fluoroorganic compound Title Simultaneous nitrification and phosphate removal by bioaugmented aerobic granules treating a fluoroorganic compound Author Duque, Anouk F. (Universidade Católica Portuguesa) Bessa, Vânia S. (Universidade Católica Portuguesa) van Dongen, L.G.J.M. (TU Delft BT/Environmental Biotechnology) de Kreuk, M.K. (TU Delft Sanitary Engineering) Mesquita, Raquel B.R. (Universidade Católica Portuguesa) Rangel, António O.S.S. (Universidade Católica Portuguesa) van Loosdrecht, Mark C.M. (TU Delft BT/Environmental Biotechnology) Castro, Paula M.L. (Universidade Católica Portuguesa) Date 2021 Abstract The presence of toxic compounds in wastewater can cause problems for organic matter and nutrient removal. In this study, the long-term effect of a model xenobiotic, 2-fluorophenol (2-FP), on ammonia-oxidizing bacteria (AOB), nitrite oxidizing bacteria (NOB) and phosphate accumulating organisms (PAO) in aerobic granular sludge was investigated. Phosphate (P) and ammonium (N) removal efficiencies were high (>93%) and, after bioaugmentation with 2-FP degrading strain FP1, 2-FP was completely degraded. Neither N nor P removal were affected by 50 mg L1 of 2-FP in the feed stream. Changes in the aerobic granule bacterial communities were followed. Numerical analysis of the denaturing gradient gel electrophoresis (DGGE) profiles showed low diversity for the ammonia monooxygenase (amoA) gene with an even distribution of species. PAOs, including denitrifying PAO (dPAO), and AOB were present in the 2-FP degrading granules, although dPAO population decreased throughout the 444 days reactor operation. The results demonstrated that the aerobic granules bioaugmented with FP1 strain successfully removed N, P and 2-FP simultaneously. Subject 2-fluorophenol (2-FP)Aerobic granular sludgeAmmonia-oxidizing bacteria (AOB)Microbial population dynamicsNitrite oxidizing bacteria (NOB)Phosphate accumulating organisms (PAO) To reference this document use: http://resolver.tudelft.nl/uuid:38f40237-151b-4436-9d59-21b11ad86b8e DOI https://doi.org/10.2166/wst.2021.142 ISSN 0273-1223 Source Water Science and Technology, 83 (10), 2404-2413 Part of collection Institutional Repository Document type journal article Rights © 2021 Anouk F. Duque, Vânia S. Bessa, L.G.J.M. van Dongen, M.K. de Kreuk, Raquel B.R. Mesquita, António O.S.S. Rangel, Mark C.M. van Loosdrecht, Paula M.L. Castro Files PDF wst083102404.pdf 554.69 KB Close viewer /islandora/object/uuid:38f40237-151b-4436-9d59-21b11ad86b8e/datastream/OBJ/view