Print Email Facebook Twitter The archaeal division protein CdvB1 assembles into polymers that are depolymerized by CdvC Title The archaeal division protein CdvB1 assembles into polymers that are depolymerized by CdvC Author Blanch Jover, A. (TU Delft BN/Cees Dekker Lab; Kavli institute of nanoscience Delft) De Franceschi, N. (TU Delft BN/Cees Dekker Lab; Kavli institute of nanoscience Delft) Fenel, Daphna (Université Grenoble Alpes) Weissenhorn, Winfried (Université Grenoble Alpes) Dekker, C. (TU Delft BN/Cees Dekker Lab; Kavli institute of nanoscience Delft) Date 2022 Abstract The Cdv proteins constitute the cell division system of the Crenarchaea, a machinery closely related to the ESCRT system of eukaryotes. Using a combination of TEM imaging and biochemical assays, we here present an in vitro study of Metallosphaera sedula CdvB1, the Cdv protein that is believed to play a major role in the constricting ring that drives cell division in the Crenarchaea. We show that CdvB1 self-assembles into filaments that are depolymerized by the Vps4-homolog ATPase CdvC. Furthermore, we find that CdvB1 binds to negatively charged lipid membranes and can be detached from the membrane by the action of CdvC. Our findings provide novel insight into one of the main components of the archaeal cell division machinery. Subject ArchaeaCdv systemCdvB1cell divisionESCRT-III To reference this document use: http://resolver.tudelft.nl/uuid:3d7bff96-e838-4ba2-a10e-36ef47fe5bc3 DOI https://doi.org/10.1002/1873-3468.14324 ISSN 0014-5793 Source FEBS Letters, 596 (7), 958-969 Part of collection Institutional Repository Document type journal article Rights © 2022 A. Blanch Jover, N. De Franceschi, Daphna Fenel, Winfried Weissenhorn, C. Dekker Files PDF FEBS_Letters_2022_Blanch_ ... zed_by.pdf 2.42 MB Close viewer /islandora/object/uuid:3d7bff96-e838-4ba2-a10e-36ef47fe5bc3/datastream/OBJ/view