Print Email Facebook Twitter Leloir Glycosyltransferases in Applied Biocatalysis Title Leloir Glycosyltransferases in Applied Biocatalysis: A Multidisciplinary Approach Author Mestrom, L. (TU Delft BT/Biocatalysis) Przypis, Marta (Silesian University of Technology) Kowalczykiewicz, Daria (Silesian University of Technology) Pollender, André (University of Technology Bergakademie Freiberg) Kumpf, Antje (University of Technology Bergakademie Freiberg; Ruhr-Universität Bochum) Marsden, S.R. (TU Delft BT/Biocatalysis) Szymańska, Katarzyna Hanefeld, U. (TU Delft BT/Biocatalysis) Hagedoorn, P.L. (TU Delft BT/Biocatalysis) Date 2019 Abstract Enzymes are nature's catalyst of choice for the highly selective and efficient coupling of carbohydrates. Enzymatic sugar coupling is a competitive technology for industrial glycosylation reactions, since chemical synthetic routes require extensive use of laborious protection group manipulations and often lack regio- and stereoselectivity. The application of Leloir glycosyltransferases has received considerable attention in recent years and offers excellent control over the reactivity and selectivity of glycosylation reactions with unprotected carbohydrates, paving the way for previously inaccessible synthetic routes. The development of nucleotide recycling cascades has allowed for the efficient production and reuse of nucleotide sugar donors in robust one-pot multi-enzyme glycosylation cascades. In this way, large glycans and glycoconjugates with complex stereochemistry can be constructed. With recent advances, LeLoir glycosyltransferases are close to being applied industrially in multi-enzyme, programmable cascade glycosylations. Subject applied biocatalysiscarbohydratechemoenzymatic synthesisenzyme cascadesglycosyltransferaseLeloirnucleotidesugar chemistry To reference this document use: http://resolver.tudelft.nl/uuid:440db8c0-a2b9-4eee-91b2-e3d5030ef54b DOI https://doi.org/10.3390/ijms20215263 ISSN 1422-0067 Source International Journal of Molecular Sciences (Online), 20 (21) Part of collection Institutional Repository Document type review Rights © 2019 L. Mestrom, Marta Przypis, Daria Kowalczykiewicz, André Pollender, Antje Kumpf, S.R. Marsden, Katarzyna Szymańska, U. Hanefeld, P.L. Hagedoorn, More Authors Files PDF ijms_20_05263_v2.pdf 4.48 MB Close viewer /islandora/object/uuid:440db8c0-a2b9-4eee-91b2-e3d5030ef54b/datastream/OBJ/view