Print Email Facebook Twitter Reinitiated viral RNA-dependent RNA polymerase resumes replication at a reduced rate Title Reinitiated viral RNA-dependent RNA polymerase resumes replication at a reduced rate Author Vilfan, I.D. Candelli, A. Hage, S. Aalto, A.P. Poranen, M.M. Bamford, D.H. Dekker, N.H. Faculty Applied Sciences Department Kavli Institute of Nanoscience Date 2008-11-05 Abstract RNA-dependent RNA polymerases (RdRP) form an important class of enzymes that is responsible for genome replication and transcription in RNA viruses and involved in the regulation of RNA interference in plants and fungi. The RdRP kinetics have been extensively studied, but pausing, an important regulatory mechanism for RNA polymerases that has also been implicated in RNA recombination, has not been considered. Here, we report that RdRP experience a dramatic, long-lived decrease in its elongation rate when it is reinitiated following stalling. The rate decrease has an intriguingly weak temperature dependence, is independent of both the nucleotide concentration during stalling and the length of the RNA transcribed prior to stalling; however it is sensitive to RNA structure. This allows us to delineate the potential factors underlying this irreversible conversion of the elongation complex to a less active mode. To reference this document use: http://resolver.tudelft.nl/uuid:5018c9e7-28ac-49cb-bb7d-b86ebe41d2a5 Publisher Oxford University Press ISSN 0305-1048 Source Nucleic Acids Research, 36 (22), 2008 Part of collection Institutional Repository Document type journal article Rights (c) 2008 The Author(s) Files PDF Vilfan_2008.pdf 692.81 KB PDF Vilfan_2008-_suppl._material.pdf 2.51 MB Close viewer /islandora/object/uuid:5018c9e7-28ac-49cb-bb7d-b86ebe41d2a5/datastream/OBJ1/view