Microwell-enhanced optical rapid antibiotic susceptibility testing of single bacteria

Microwell-enhanced optical rapid antibiotic susceptibility testing of single bacteria

Roslon, I.E. (TU Delft Dynamics of Micro and Nano Systems; SoundCell B.V.)
Japaridze, A. (TU Delft Dynamics of Micro and Nano Systems; SoundCell B.V.)
Rodenhuis, Stef (Student TU Delft)
Hamoen, Lieke (Student TU Delft)
Ghatkesar, M.K. (TU Delft Micro and Nano Engineering)
Steeneken, P.G. (TU Delft Dynamics of Micro and Nano Systems)
Dekker, C. (TU Delft BN/Cees Dekker Lab)
Alijani, F. (TU Delft Dynamics of Micro and Nano Systems)

2023

Bacteria that are resistant to antibiotics present an increasing burden on healthcare. To address this emerging crisis, novel rapid antibiotic susceptibility testing (AST) methods are eagerly needed. Here, we present an optical AST technique that can determine the bacterial viability within 1 h down to a resolution of single bacteria. The method is based on measuring intensity fluctuations of a reflected laser focused on a bacterium in reflective microwells. Using numerical simulations, we show that both refraction and absorption of light by the bacterium contribute to the observed signal. By administering antibiotics that kill the bacteria, we show that the variance of the detected fluctuations vanishes within 1 h, indicating the potential of this technique for rapid sensing of bacterial antibiotic susceptibility. We envisage the use of this method for massively parallelizable AST tests and fast detection of drug-resistant pathogens.

Medical Microbiology
Microbiology

http://resolver.tudelft.nl/uuid:53a7d176-6e40-4fe6-88de-1d4e10ba5eed

https://doi.org/10.1016/j.isci.2023.108268

2589-0042

iScience, 26 (11)

Institutional Repository

journal article

© 2023 I.E. Roslon, A. Japaridze, Stef Rodenhuis, Lieke Hamoen, M.K. Ghatkesar, P.G. Steeneken, C. Dekker, F. Alijani