Print Email Facebook Twitter SMC complexes can traverse physical roadblocks bigger than their ring size Title SMC complexes can traverse physical roadblocks bigger than their ring size Author Pradhan, B. (TU Delft BN/Cees Dekker Lab; TU Delft BN/Bionanoscience; Kavli institute of nanoscience Delft) Barth, R. (TU Delft BN/Cees Dekker Lab; Kavli institute of nanoscience Delft) Kim, E. (TU Delft BN/Cees Dekker Lab; TU Delft BN/Bionanoscience; Kavli institute of nanoscience Delft) van Laar, T. (TU Delft BN/Nynke Dekker Lab; Kavli institute of nanoscience Delft) Yang, W.W.W. (TU Delft BN/Cees Dekker Lab; TU Delft BN/Bionanoscience; Kavli institute of nanoscience Delft) Ryu, J.K. (TU Delft BN/Cees Dekker Lab; Kavli institute of nanoscience Delft) van der Torre, J. (TU Delft BN/Cees Dekker Lab; Kavli institute of nanoscience Delft) Peters, Jan Michael (Research Institute of Molecular Pathology, Vienna) Dekker, C. (TU Delft BN/Cees Dekker Lab; TU Delft BN/Bionanoscience; Kavli institute of nanoscience Delft) Department BN/Bionanoscience Date 2022 Abstract Ring-shaped structural maintenance of chromosomes (SMC) complexes like condensin and cohesin extrude loops of DNA. It remains, however, unclear how they can extrude DNA loops in chromatin that is bound with proteins. Here, we use in vitro single-molecule visualization to show that nucleosomes, RNA polymerase, and dCas9 pose virtually no barrier to loop extrusion by yeast condensin. We find that even DNA-bound nanoparticles as large as 200 nm, much bigger than the SMC ring size, also translocate into DNA loops during extrusion by condensin and cohesin. This even occurs for a single-chain version of cohesin in which the ring-forming subunits are covalently linked and cannot open to entrap DNA. The data show that SMC-driven loop extrusion has surprisingly little difficulty in accommodating large roadblocks into the loop. The findings also show that the extruded DNA does not pass through the SMC ring (pseudo)topologically, hence pointing to a nontopological mechanism for DNA loop extrusion. Subject cohesincondensinCP: Molecular biologydCas9DNA loop extrusionmechanismnucleosomesRNA polymeraseroadblocksSMCtopology To reference this document use: http://resolver.tudelft.nl/uuid:554bbfe8-02be-4527-96d3-e70b979d1b98 DOI https://doi.org/10.1016/j.celrep.2022.111491 ISSN 2211-1247 Source Cell Reports, 41 (3) Part of collection Institutional Repository Document type journal article Rights © 2022 B. Pradhan, R. Barth, E. Kim, T. van Laar, W.W.W. Yang, J.K. Ryu, J. van der Torre, Jan Michael Peters, C. Dekker, More Authors Files PDF 1_s2.0_S2211124722013419_main.pdf 11.34 MB Close viewer /islandora/object/uuid:554bbfe8-02be-4527-96d3-e70b979d1b98/datastream/OBJ/view