Print Email Facebook Twitter Approaches for bacteriophage genome engineering Title Approaches for bacteriophage genome engineering Author Mahler, M.S. (TU Delft BN/Stan Brouns Lab; University of Otago) Martins Costa, A.R. (TU Delft BN/Stan Brouns Lab; Kavli institute of nanoscience Delft) van Beljouw, S.P.B. (TU Delft BN/Stan Brouns Lab; Kavli institute of nanoscience Delft) Fineran, Peter C. (University of Otago) Brouns, S.J.J. (TU Delft BN/Stan Brouns Lab; Kavli institute of nanoscience Delft) Date 2023 Abstract In recent years, bacteriophage research has been boosted by a rising interest in using phage therapy to treat antibiotic-resistant bacterial infections. In addition, there is a desire to use phages and their unique proteins for specific biocontrol applications and diagnostics. However, the ability to manipulate phage genomes to understand and control gene functions, or alter phage properties such as host range, has remained challenging due to a lack of universal selectable markers. Here, we discuss the state-of-the-art techniques to engineer and select desired phage genomes using advances in cell-free methodologies and clustered regularly interspaced short palindromic repeats-CRISPR associated protein (CRISPR-Cas) counter-selection approaches. Subject CRISPR-Cashomologous recombinationphage engineeringrebootingrecombineering To reference this document use: http://resolver.tudelft.nl/uuid:5e1f276c-92a2-4600-89ad-1fa1a5dec79f DOI https://doi.org/10.1016/j.tibtech.2022.08.008 ISSN 0167-7799 Source Trends in Biotechnology, 41 (5), 669-685 Part of collection Institutional Repository Document type review Rights © 2023 M.S. Mahler, A.R. Martins Costa, S.P.B. van Beljouw, Peter C. Fineran, S.J.J. Brouns Files PDF 1_s2.0_S0167779922002268_main.pdf 1.73 MB Close viewer /islandora/object/uuid:5e1f276c-92a2-4600-89ad-1fa1a5dec79f/datastream/OBJ/view