Print Email Facebook Twitter Mimicked Mixing-Induced Heterogeneities of Industrial Bioreactors Stimulate Long-Lasting Adaption Programs in Ethanol-Producing Yeasts Title Mimicked Mixing-Induced Heterogeneities of Industrial Bioreactors Stimulate Long-Lasting Adaption Programs in Ethanol-Producing Yeasts Author Minden, Steven (University of Stuttgart) Aniolek, Maria (University of Stuttgart) Noorman, H.J. (TU Delft BT/Bioprocess Engineering; DSM) Takors, Ralf (University of Stuttgart) Date 2023 Abstract Commercial-scale bioreactors create an unnatural environment for microbes from an evolutionary point of view. Mixing insufficiencies expose individual cells to fluctuating nutrient concentrations on a second-to-minute scale while transcriptional and translational capacities limit the microbial adaptation time from minutes to hours. This mismatch carries the risk of inadequate adaptation effects, especially considering that nutrients are available at optimal concentrations on average. Consequently, industrial bioprocesses that strive to maintain microbes in a phenotypic sweet spot, during lab-scale development, might suffer performance losses when said adaptive misconfigurations arise during scale-up. Here, we investigated the influence of fluctuating glucose availability on the gene-expression profile in the industrial yeast Ethanol Red™. The stimulus-response experiment introduced 2 min glucose depletion phases to cells growing under glucose limitation in a chemostat. Even though Ethanol Red™ displayed robust growth and productivity, a single 2 min depletion of glucose transiently triggered the environmental stress response. Furthermore, a new growth phenotype with an increased ribosome portfolio emerged after complete adaptation to recurring glucose shortages. The results of this study serve a twofold purpose. First, it highlights the necessity to consider the large-scale environment already at the experimental development stage, even when process-related stressors are moderate. Second, it allowed the deduction of strain engineering guidelines to optimize the genetic background of large-scale production hosts. Subject bioreactorEthanol Red™Saccharomyces cerevisiaescale-downscale-upstimulus–response experimentsubstrate gradienttranscriptomics To reference this document use: http://resolver.tudelft.nl/uuid:5e615c6f-e29a-4292-aa8e-d68ea998df0b DOI https://doi.org/10.3390/genes14050997 ISSN 2073-4425 Source Genes, 14 (5) Part of collection Institutional Repository Document type journal article Rights © 2023 Steven Minden, Maria Aniolek, H.J. Noorman, Ralf Takors Files PDF genes_14_00997_v2.pdf 3.66 MB Close viewer /islandora/object/uuid:5e615c6f-e29a-4292-aa8e-d68ea998df0b/datastream/OBJ/view