Print Email Facebook Twitter Opto-electronic feedback control of membrane potential for real-time control of action potentials Title Opto-electronic feedback control of membrane potential for real-time control of action potentials Author Ördög, Balázs (Leiden University Medical Center) De Coster, Tim (Leiden University Medical Center) Dekker, Sven O. (Leiden University Medical Center) Bart, Cindy I. (Leiden University Medical Center) Zhang, Juan (Leiden University Medical Center) Boink, Gerard J.J. (Universiteit van Amsterdam) Bax, Wilhelmina H. (Leiden University Medical Center) Deng, Shanliang (TU Delft Electronic Components, Technology and Materials; Leiden University Medical Center) den Ouden, B.L. (TU Delft Electronic Components, Technology and Materials) de Vries, Antoine A.F. (Leiden University Medical Center) Pijnappels, Daniël A. (Leiden University Medical Center) Date 2023 Abstract To unlock new research possibilities by acquiring control of action potential (AP) morphologies in excitable cells, we developed an opto-electronic feedback loop-based system integrating cellular electrophysiology, real-time computing, and optogenetic approaches and applied it to monolayers of heart muscle cells. This allowed accurate restoration and preservation of cardiac AP morphologies in the presence of electrical perturbations of different origin in an unsupervised, self-regulatory manner, without any prior knowledge of the disturbance. Moreover, arbitrary AP waveforms could be enforced onto these cells. Collectively, these results set the stage for the refinement and application of opto-electronic control systems to enable in-depth investigation into the regulatory role of membrane potential in health and disease. Subject action potentialmembrane potentialcellular electrophysiologyfeedback loopreal-time computingoptogeneticsClosed-loop controlsystem integrationheart muscle cells To reference this document use: http://resolver.tudelft.nl/uuid:6bc5af25-aaf5-41bc-9708-db555b3933bb DOI https://doi.org/10.1016/j.crmeth.2023.100671 ISSN 2667-2375 Source Cell Reports Methods, 3 (12) Part of collection Institutional Repository Document type journal article Rights © 2023 Balázs Ördög, Tim De Coster, Sven O. Dekker, Cindy I. Bart, Juan Zhang, Gerard J.J. Boink, Wilhelmina H. Bax, Shanliang Deng, B.L. den Ouden, Antoine A.F. de Vries, Daniël A. Pijnappels Files PDF 1_s2.0_S2667237523003508_main.pdf 4.8 MB Close viewer /islandora/object/uuid:6bc5af25-aaf5-41bc-9708-db555b3933bb/datastream/OBJ/view