Print Email Facebook Twitter Weak Acid Permeation in Synthetic Lipid Vesicles and Across the Yeast Plasma Membrane Title Weak Acid Permeation in Synthetic Lipid Vesicles and Across the Yeast Plasma Membrane Author Gabba, Matteo (Rijksuniversiteit Groningen) Frallicciardi, Jacopo (Rijksuniversiteit Groningen) van ’t Klooster, Joury (Rijksuniversiteit Groningen) Henderson, Ryan (Rijksuniversiteit Groningen) Syga, Łukasz (Rijksuniversiteit Groningen) Mans, R. (TU Delft BT/Industriele Microbiologie) van Maris, A.J.A. (TU Delft BT/Industriele Microbiologie; KTH Royal Institute of Technology) Poolman, Bert (Rijksuniversiteit Groningen) Date 2020 Abstract We present a fluorescence-based approach for determination of the permeability of small molecules across the membranes of lipid vesicles and living cells. With properly designed experiments, the method allows us to assess the membrane physical properties both in vitro and in vivo. We find that the permeability of weak acids increases in the order of benzoic > acetic > formic > lactic, both in synthetic lipid vesicles and the plasma membrane of Saccharomyces cerevisiae, but the permeability is much lower in yeast (one to two orders of magnitude). We observe a relation between the molecule permeability and the saturation of the lipid acyl chain (i.e., lipid packing) in the synthetic lipid vesicles. By analyzing wild-type yeast and a manifold knockout strain lacking all putative lactic acid transporters, we conclude that the yeast plasma membrane is impermeable to lactic acid on timescales up to ∼2.5 h. To reference this document use: http://resolver.tudelft.nl/uuid:6cfbafb6-509b-4175-b7b4-1745e5353b36 DOI https://doi.org/10.1016/j.bpj.2019.11.3384 ISSN 0006-3495 Source Biophysical Journal, 118 (2), 422-434 Part of collection Institutional Repository Document type journal article Rights © 2020 Matteo Gabba, Jacopo Frallicciardi, Joury van ’t Klooster, Ryan Henderson, Łukasz Syga, R. Mans, A.J.A. van Maris, Bert Poolman Files PDF 1_s2.0_S0006349519343413_main.pdf 1.1 MB Close viewer /islandora/object/uuid:6cfbafb6-509b-4175-b7b4-1745e5353b36/datastream/OBJ/view