Print Email Facebook Twitter The impact of lipid handling and phase distribution on the acoustic behavior of microbubbles Title The impact of lipid handling and phase distribution on the acoustic behavior of microbubbles Author Langeveld, Simone A.G. (Erasmus MC) Beekers, Inés (Erasmus MC) Collado Lara, G. (Erasmus MC) van der Steen, A.F.W. (TU Delft ImPhys/Medical Imaging; Erasmus MC) de Jong, N. (TU Delft ImPhys/Medical Imaging; Erasmus MC) Kooiman, Klazina (Erasmus MC) Date 2021 Abstract Phospholipid-coated microbubbles are ultrasound contrast agents that can be employed for ultrasound molecular imaging and drug delivery. For safe and effective implementation, microbubbles must respond uniformly and predictably to ultrasound. Therefore, we investigated how lipid handling and phase distribution affected the variability in the acoustic behavior of microbubbles. Cholesterol was used to modify the lateral molecular packing of 1,2-distearoyl-sn-glycero-3phosphocholine (DSPC)-based microbubbles. To assess the effect of lipid handling, microbubbles were produced by a direct method, i.e., lipids directly dispersed in an aqueous medium or indirect method, i.e., lipids first dissolved in an organic solvent. The lipid phase and ligand distribution in the microbubble coating were investigated using confocal microscopy, and the acoustic response was recorded with the Brandaris 128 ultra-high-speed camera. In microbubbles with 12 mol% cholesterol, the lipids were miscible and all in the same phase, which resulted in more buckle formation, lower shell elasticity and higher shell viscosity. Indirect DSPC microbubbles had a more uniform response to ultrasound than direct DSPC and indirect DSPC-cholesterol microbubbles. The difference in lipid handling between direct and indirect DSPC microbubbles significantly affected the acoustic behavior. Indirect DSPC microbubbles are the most promising candidate for ultrasound molecular imaging and drug delivery applications. Subject Acoustic responseCholesterolLigand distributionLipid phaseMicrobubblePhospholipid coatingUltrasound contrast agents To reference this document use: http://resolver.tudelft.nl/uuid:b538112b-a2fa-4b07-8c41-2465e4702dbb DOI https://doi.org/10.3390/pharmaceutics13010119 ISSN 1543-8384 Source Molecular Pharmaceutics, 13 (1), 1-21 Part of collection Institutional Repository Document type journal article Rights © 2021 Simone A.G. Langeveld, Inés Beekers, G. Collado Lara, A.F.W. van der Steen, N. de Jong, Klazina Kooiman Files PDF pharmaceutics_13_00119.pdf 2.51 MB Close viewer /islandora/object/uuid:b538112b-a2fa-4b07-8c41-2465e4702dbb/datastream/OBJ/view