Title
CRISPR-controlled proteases
Author
van Beljouw, S.P.B. (TU Delft BN/Stan Brouns Lab; Kavli institute of nanoscience Delft)
Brouns, S.J.J. (TU Delft BN/Stan Brouns Lab; Kavli institute of nanoscience Delft) 
Date
2024
Abstract
With the discovery of CRISPR-controlled proteases, CRISPR–Cas has moved beyond mere nucleic acid targeting into the territory of targeted protein cleavage. Here, we review the understanding of Craspase, the best-studied member of the growing CRISPR RNA-guided protease family. We recollect the original bioinformatic prediction and early experimental characterizations; evaluate some of the mechanistic structural intricacies and emerging biotechnology; discuss open questions and unexplained mysteries; and indicate future directions for the rapidly moving field of the CRISPR proteases.
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http://resolver.tudelft.nl/uuid:c15129c1-4e9e-4a0a-9932-ea913082d16d
DOI
https://doi.org/10.1042/BST20230962
Embargo date
2024-08-09
ISSN
0300-5127
Source
Biochemical Society Transactions, 52
Part of collection
Institutional Repository
Document type
review
Rights
© 2024 S.P.B. van Beljouw, S.J.J. Brouns