Print Email Facebook Twitter Vitamin requirements and biosynthesis in Saccharomyces cerevisiae Title Vitamin requirements and biosynthesis in Saccharomyces cerevisiae Author Perli, T. (TU Delft BT/Industriele Microbiologie) Wronska, A.K. (TU Delft BT/Industriele Microbiologie) Ortiz Merino, R.A. (TU Delft BT/Industriele Microbiologie) Pronk, J.T. (TU Delft BT/Biotechnologie) Daran, J.G. (TU Delft BT/Industriele Microbiologie) Department BT/Biotechnologie Date 2020 Abstract Chemically defined media for yeast cultivation (CDMY) were developed to support fast growth, experimental reproducibility, and quantitative analysis of growth rates and biomass yields. In addition to mineral salts and a carbon substrate, popular CDMYs contain seven to nine B-group vitamins, which are either enzyme cofactors or precursors for their synthesis. Despite the widespread use of CDMY in fundamental and applied yeast research, the relation of their design and composition to the actual vitamin requirements of yeasts has not been subjected to critical review since their first development in the 1940s. Vitamins are formally defined as essential organic molecules that cannot be synthesized by an organism. In yeast physiology, use of the term “vitamin” is primarily based on essentiality for humans, but the genome of the Saccharomyces cerevisiae reference strain S288C harbours most of the structural genes required for synthesis of the vitamins included in popular CDMY. Here, we review the biochemistry and genetics of the biosynthesis of these compounds by S. cerevisiae and, based on a comparative genomics analysis, assess the diversity within the Saccharomyces genus with respect to vitamin prototrophy. Subject fermentationgrowth requirementsSaccharomyces cerevisiaesynthetic mediavitamin biosynthesis To reference this document use: http://resolver.tudelft.nl/uuid:c9ef9a8d-81b6-4bda-8352-69402be6a8b5 DOI https://doi.org/10.1002/yea.3461 ISSN 0749-503X Source Yeast, 37 (4), 283-304 Part of collection Institutional Repository Document type journal article Rights © 2020 T. Perli, A.K. Wronska, R.A. Ortiz Merino, J.T. Pronk, J.G. Daran Files PDF Perli_et_al_2020_Yeast.pdf 3.19 MB Close viewer /islandora/object/uuid:c9ef9a8d-81b6-4bda-8352-69402be6a8b5/datastream/OBJ/view