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Puiman, L. (author)One of the major challenges mankind faces nowadays is combating climate change. A substantial fraction of greenhouse gases are released by industrial processes, as steelmaking, (oil)refinery and waste processing. Emissions from these processes can partly be prevented with a recently developed technology called gas fermentation. Within this...doctoral thesis 2024
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Jankovic, T.J. (author), Straathof, Adrie J.J. (author), McGregor, Ian R. (author), Kiss, A.A. (author)Distillation is the most used separation technology at industrial-scale, but using distillation in bio-based processes (e.g. fermentation processes to produce bioethanol) is quite challenging when mild temperatures are needed to keep the microbes alive. Vacuum distillation can be used to perform evaporation at low temperatures, but setting a...journal article 2024
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Puiman, L. (author), Almeida Benalcazar, E.F. (author), Picioreanu, Cristian (author), Noorman, H.J. (author), Haringa, C. (author)Gradients in dissolved gas concentrations are expected to affect the performance of large reactors for anaerobic gas (CO, H2, CO2) fermentation. To study how these gradients, and the dissolved gas concentration level itself, influence the productivity of the desired product ethanol and the product spectrum of C. autoethanogenum, we coupled a CFD...journal article 2024
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Almeida Benalcazar, E.F. (author), Mondini, Camille (author), Bruant, Guillaume (author), Tremblay, Julien (author), Weissbrodt, D.G. (author), Mockaitis, Gustavo (author)BACKGROUND: Carboxylates such as volatile fatty acids (VFA) can be produced by acidogenic fermentation (AF) of dairy wastes including cheese whey, a massive residue produced at 160.67 million m<sup>3</sup> of which 42% are not valorized and impact the environment. In mixed-culture fermentations, selection pressures can favor AF and halt...journal article 2024
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Atasoy, M. (author), Scott Jr, William T. (author), Regueira, Alberte (author), Mauricio-Iglesias, Miguel (author), Schaap, Peter J. (author), Smidt, Hauke (author)In recent years, there has been growing interest in harnessing anaerobic digestion technology for resource recovery from waste streams. This approach has evolved beyond its traditional role in energy generation to encompass the production of valuable carboxylic acids, especially volatile fatty acids (VFAs) like acetic acid, propionic acid,...review 2024
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Jankovic, T.J. (author), Straathof, Adrie J.J. (author), Kiss, A.A. (author)PDO (1,3-propanediol) is a platform chemical that is obtained by petrochemical routes and by fermentation. The latter needs relatively complex downstream processing after fermentation, due to the modest concentration of the high-boiling product, and the presence of microorganisms and many impurities in the fermentation medium. This novel...journal article 2024
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Jankovic, T.J. (author), Straathof, Adrie J.J. (author), Kiss, A.A. (author)Even though industrial biotechnology is successfully used for the production of some chemicals, for many other chemicals it is not yet competitive with conventional petrochemical production. Usually, fermentation as well as downstream processing requires improvement. Downstream processing has to deal with low product concentrations,...journal article 2024
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Almeida Benalcazar, E.F. (author)The mitigation of global warming requires an urgent shift from the fossil fuel-based productive matrix currently in place. Technological platforms are being developed to reduce the amount of carbon of fossil origin, which is emitted to the atmosphere as a side-product from the production of energy. Gas mixtures containing CO, H2 and CO2 are...doctoral thesis 2023
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Allaart, M.T. (author)One of the main challenges society currently deals with is the depletion of fossil fuels. To navigate this issue, we must embrace the concept of circularity and turn waste into a resource. Waste streams are omnifarious and their conversion into new chemical building blocks is not always trivial. Luckily, we can take a look at nature’s problem...doctoral thesis 2023
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van Aalst, A.C.A. (author)The production of ethanol by the yeast Saccharomyces cerevisiae remains the process in industrial biotechnology with the largest product volume (ca. 100 million litres annually in 2022). Carbon losses occur due to the formation of biomass and glycerol, which can account for at least 8% of the product. Under anaerobic conditions, formation of...doctoral thesis 2023
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Christofi, Andreas (author)Dating back to 10,000 B.C.E., fermentation has served as a vital tool primarily for food preservation. In the nineteenth century, Louis Pasteur put forth the proposition that the occurrence of fermentation is predominantly facilitated by the presence of microorganisms. Pasteur’s discovery spurred comprehensive studies on microorganisms,...master thesis 2023
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Lehnerdt, Luise (author)The global production of textiles carries a significant share in global greenhouse gas emissions, with a demand projected to grow. Especially for scope 3 emissions, materials play a significant role, with polyethylene terephthalate (PET) as a dominating fibre material with unique properties. Virgin PET based on fossil oil as the conventional...master thesis 2023
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Chowdhary, Kshitij (author)Of all the greenhouse gases (GHGs), carbon dioxide (CO2) has been the target of most climate recovery efforts as it is the most abundantly emitted GHG by mass. In fact, in 2015 a legally binding international treaty was adopted by 196 parties in Paris, France to constrain the anthropogenic warming to 1.5-2.0˚C above the pre-industrial level. In...master thesis 2023
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Jankovic, T.J. (author), Straathof, Adrie J.J. (author), Kiss, A.A. (author)BACKGROUND: Isopropanol and acetone production by syngas fermentation is a promising alternative to conventional fossil carbon-dependent production. However, this alternative technology has not yet been scaled up to an industrial level owing to the relatively low product concentrations (about 5 wt% in total). This original research aims to...journal article 2023
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Elahinik, A. (author), Li, L.H (author), Pabst, Martin (author), Abbas, B.A. (author), Xevgenos, Dimitris (author), van Loosdrecht, Mark C.M. (author), Pronk, M. (author)Enhanced biological phosphate removal and aerobic sludge granulation are commonly studied with fatty acids as substrate. Fermentative substrates such as glucose have received limited attention. In this work, glucose conversion by aerobic granular sludge and its impact on phosphate removal was studied. Long-term stable phosphate removal and...journal article 2023
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Jankovic, T.J. (author), Straathof, Adrie J.J. (author), Kiss, A.A. (author)Syngas fermentation is used industrially to produce diluted bioethanol (about 1–6 wt%). This research study proposes a novel downstream process that recovers bioethanol in an energy-efficient and cost-effective manner, improves fermentation yield by recycling all fermentation broth components (microbes, acetate and water), and is designed for...journal article 2023
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Puiman, L. (author), Almeida Benalcazar, E.F. (author), Picioreanu, C. (author), Noorman, H.J. (author), Haringa, C. (author)In large-scale syngas fermentation, strong gradients in dissolved gas (CO, H<sub>2</sub>) concentrations are very likely to occur due to locally varying mass transfer and convection rates. Using Euler-Lagrangian CFD simulations, we analyzed these gradients in an industrial-scale external-loop gas-lift reactor (EL-GLR) for a wide range of...journal article 2023
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Cerruti, M. (author), Crosset-Perrotin, G. (author), Ananth, Mythili (author), Rombouts, Julius Laurens (author), Weissbrodt, D.G. (author)Fermentative chemoorganoheterotrophic bacteria (FCB) and purple photoorganoheterotrophic bacteria (PPB) are two interesting microbial guilds to process carbohydrate-rich wastewaters. Their metabolic interactions have been studied in pure cultures or co-cultures, but little is known about mixed cultures. We studied the effect of reactor...journal article 2023
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Wei, P. (author), Haringa, C. (author), Portela, L. (author), Noorman, H.J. (author)This study focuses on the metabolic impacts of simultaneous glucose and oxygen concentration gradients on penicillin production in an industrial-scale fermentor, using the computational fluid dynamics-cellular reaction dynamics approach. Inclusion of oxygen-coupling considerably impacts the glucose uptake and resulting penicillin productivity...journal article 2023
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van Aalst, Aafke C.A. (author), Geraats, Ellen H. (author), Martini, M.L.A. (author), Mans, R. (author), Pronk, J.T. (author)In anaerobic Saccharomyces cerevisiae cultures, NADH (reduced form of nicotinamide adenine dinucleotide)-cofactor balancing by glycerol formation constrains ethanol yields. Introduction of an acetate-to-ethanol reduction pathway based on heterologous acetylating acetaldehyde dehydrogenase (A-ALD) can replace glycerol formation as 'redox-sink'...journal article 2023