Print Email Facebook Twitter Structure-Based Redesign of a Methanol Oxidase into an “Aryl Alcohol Oxidase” for Enzymatic Synthesis of Aromatic Flavor Compounds Title Structure-Based Redesign of a Methanol Oxidase into an “Aryl Alcohol Oxidase” for Enzymatic Synthesis of Aromatic Flavor Compounds Author Wu, Bin (South China University of Technology) Wang, S. (Chinese Academy of Sciences; University of Chinese Academy of Sciences) Ma, Yunjian (South China University of Technology) Yuan, Shuguang (Chinese Academy of Sciences) Hollmann, F. (TU Delft BT/Biocatalysis) Wang, Yonghua (South China University of Technology) Date 2023 Abstract Alcohol oxidases (AOxs) catalyze the aerobic oxidation of alcohols to the corresponding carbonyl products (aldehydes or ketones), producing only H2O2 as the byproduct. The majority of known AOxs, however, have a strong preference for small, primary alcohols, limiting their broad applicability, e.g., in the food industry. To broaden the product scope of AOxs, we performed structure-guided enzyme engineering of a methanol oxidase from Phanerochaete chrysosporium (PcAOx). The substrate preference was extended from methanol to a broad range of benzylic alcohols by modifying the substrate binding pocket. A mutant (PcAOx-EFMH) with four substitutions exhibited improved catalytic activity toward benzyl alcohols with increased conversion and kcat toward the benzyl alcohol from 11.3 to 88.9% and from 0.5 to 2.6 s-1, respectively. The molecular basis for the change of substrate selectivity was analyzed by molecular simulation. Subject alcohol oxidaseflavor compoundsmolecular modificationsubstrate selectivity To reference this document use: http://resolver.tudelft.nl/uuid:d1f9bdaa-c33a-419c-833b-26659a40968b DOI https://doi.org/10.1021/acs.jafc.3c01069 Embargo date 2023-10-11 ISSN 0021-8561 Source Journal of Agricultural and Food Chemistry, 71 (16), 6406-6414 Bibliographical note Green Open Access added to TU Delft Institutional Repository ‘You share, we take care!’ – Taverne project https://www.openaccess.nl/en/you-share-we-take-care Otherwise as indicated in the copyright section: the publisher is the copyright holder of this work and the author uses the Dutch legislation to make this work public. Part of collection Institutional Repository Document type journal article Rights © 2023 Bin Wu, S. Wang, Yunjian Ma, Shuguang Yuan, F. Hollmann, Yonghua Wang Files PDF acs.jafc.3c01069.pdf 7.12 MB Close viewer /islandora/object/uuid:d1f9bdaa-c33a-419c-833b-26659a40968b/datastream/OBJ/view