Print Email Facebook Twitter Forcing DNA and RNA through Artifical Nanopores Title Forcing DNA and RNA through Artifical Nanopores Author Van den Hout, M. Contributor Dekker, N.J. (promotor) Faculty Applied Sciences Department Bio Nanoscience Date 2010-10-21 Abstract Biological molecules can be forced to pass through an aperture in a thin membrane of only several nanometres in diameter (a nanopore). Under the right experimental conditions, the molecules will induce a change in an ionic current flowing through the nanopore. Here, we exploit this principle to detect individual molecules of DNA and RNA and study their structural properties such as diameter and charge density. We study the translocation behaviour of these molecules in nanopores with diameters as small as the cross-sectional diameter of a single DNA molecule. In addition, we use optical tweezers to arrest the molecules inside a nanopore and locally measure and apply forces, which may ultimately be exploited for the controlled detection of local structure along a single biological molecule. Subject nanoporesRNADNAOptical Tweezersnucleic acids To reference this document use: http://resolver.tudelft.nl/uuid:f1c710e0-7954-4c26-a574-681b82867314 Embargo date 2010-10-21 ISBN 9789085930853 Part of collection Institutional Repository Document type doctoral thesis Rights (c) 2010 Van den Hout, M. Files PDF Thesis_MvdHout.pdf 9.79 MB Close viewer /islandora/object/uuid:f1c710e0-7954-4c26-a574-681b82867314/datastream/OBJ/view