Print Email Facebook Twitter Immobilization protects enzymes from plasma-mediated inactivation Title Immobilization protects enzymes from plasma-mediated inactivation Author Dirks, Tim (Ruhr-Universität Bochum) Yayci, Abdulkadir (Ruhr-Universität Bochum) Klopsch, Sabrina (Ruhr-Universität Bochum) Krewing, Marco (Ruhr-Universität Bochum) Zhang, Wuyuan (Chinese Academy of Sciences) Hollmann, F. (TU Delft BT/Biocatalysis) Bandow, Julia E. (Ruhr-Universität Bochum) Date 2023 Abstract Non-thermal plasmas are used in various applications to inactivate biological agents or biomolecules. A complex cocktail of reactive species, (vacuum) UV radiation and in some cases exposure to an electric field together cause the detrimental effects. In contrast to this disruptive property of technical plasmas, we have shown previously that it is possible to use non-thermal plasma-generated species such as H2O2 as cosubstrates in biocatalytic reactions. One of the main limitations in plasma-driven biocatalysis is the relatively short enzyme lifetime under plasma-operating conditions. This challenge could be overcome by immobilizing the enzymes on inert carrier materials. Here, we tested whether immobilization is suited to protect proteins from inactivation by plasma. To this end, using a dielectric barrier discharge device (PlasmaDerm), plasma stability was tested for five enzymes immobilized on ten different carrier materials. A comparative analysis of the treatment times needed to reduce enzyme activity of immobilized and free enzyme by 30% showed a maximum increase by a factor of 44. Covalent immobilization on a partly hydrophobic carrier surface proved most effective. We conclude from the study, that immobilization universally protects enzymes under plasma-operating conditions, paving the way for new emerging applications. Subject atmospheric plasmaenzyme protectionimmobilizationnon-thermal plasma To reference this document use: http://resolver.tudelft.nl/uuid:0052a435-e56d-43dc-9392-367b450b7378 DOI https://doi.org/10.1098/rsif.2023.0299 ISSN 1742-5689 Source Journal of the Royal Society Interface, 20 (207) Part of collection Institutional Repository Document type journal article Rights © 2023 Tim Dirks, Abdulkadir Yayci, Sabrina Klopsch, Marco Krewing, Wuyuan Zhang, F. Hollmann, Julia E. Bandow Files PDF dirks_et_al_2023_immobili ... vation.pdf 686.18 KB Close viewer /islandora/object/uuid:0052a435-e56d-43dc-9392-367b450b7378/datastream/OBJ/view