Print Email Facebook Twitter Morphological maturation of the mouse brain Title Morphological maturation of the mouse brain: An in vivo MRI and histology investigation Author Hammelrath, Luam (Max Planck Institute for Metabolism Research) Škokić, Siniša (Max Planck Institute for Metabolism Research; University of Zagreb) Khmelinskii, Artem (Leiden University Medical Center; Percuros B.V) Hess, Andreas (Friedrich-Alexander-Universität Erlangen-Nürnberg) van der Knaap, Noortje (Max Planck Institute for Metabolism Research; Radboud Universiteit Nijmegen) Staring, M. (Leiden University Medical Center) Lelieveldt, B.P.F. (TU Delft Pattern Recognition and Bioinformatics; Leiden University Medical Center) Wiedermann, Dirk (Max Planck Institute for Metabolism Research) Hoehn, Mathias (Max Planck Institute for Metabolism Research; Leiden University Medical Center; Percuros B.V) Date 2016 Abstract With the wide access to studies of selected gene expressions in transgenic animals,mice have become the dominant species as cerebral diseasemodels. Many of these studies are performed on animals of not more than eight weeks, declared as adult animals. Based on the earlier reports that full brain maturation requires at least three months in rats, there is a clear need to discern the corresponding minimal animal age to provide an “adult brain” in mice in order to avoid modulation of disease progression/therapy studies by ongoing developmental changes. For this purpose, we have studied anatomical brain alterations of mice during their first six months of age. Using T2-weighted and diffusion-weightedMRI, structural and volume changes of the brain were identified and compared with histological analysis of myelination. Mouse brain volume was found to be almost stable already at three weeks, but cortex thickness kept decreasing continuously with maximal changes during the firstthree months. Myelination is still increasing between three and six months, although most dramatic changes are over by three months. While our results emphasize that mice should be at least three months old when adult animals are needed for brain studies, preferred choice of one particular metric for future investigation goals will result in somewhat varying age windows of stabilization. Subject MRIMouse brainBrain developmentCortexMyelinationDTI To reference this document use: http://resolver.tudelft.nl/uuid:13e1f7b0-a570-46e5-a299-bcfeed05f758 DOI https://doi.org/10.1016/j.neuroimage.2015.10.009 ISSN 1053-8119 Source NeuroImage, 125, 144-152 Part of collection Institutional Repository Document type journal article Rights © 2016 Luam Hammelrath, Siniša Škokić, Artem Khmelinskii, Andreas Hess, Noortje van der Knaap, M. Staring, B.P.F. Lelieveldt, Dirk Wiedermann, Mathias Hoehn Files PDF 3518762.pdf 1.56 MB Close viewer /islandora/object/uuid:13e1f7b0-a570-46e5-a299-bcfeed05f758/datastream/OBJ/view