An organ-on-chip device with integrated charge sensors and recording microelectrodes

An organ-on-chip device with integrated charge sensors and recording microelectrodes

Aydogmus, H. (TU Delft EKL Processing)
Hu, M. (TU Delft Biomechanical Engineering; Leiden University Medical Center)
Ivancevic, Lovro
Frimat, Jean Philippe (Leiden University Medical Center)
van den Maagdenberg, Arn M.J.M. (Leiden University Medical Center)
Sarro, Pasqualina M (TU Delft Electronic Components, Technology and Materials)
Mastrangeli, Massimo (TU Delft Electronic Components, Technology and Materials)

Biomechanical Engineering

2023

Continuous monitoring of tissue microphysiology is a key enabling feature of the organ-on-chip (OoC) approach for in vitro drug screening and disease modeling. Integrated sensing units are particularly convenient for microenvironmental monitoring. However, sensitive in vitro and real-time measurements are challenging due to the inherently small size of OoC devices, the characteristics of commonly used materials, and external hardware setups required to support the sensing units. Here we propose a silicon-polymer hybrid OoC device that encompasses transparency and biocompatibility of polymers at the sensing area, and has the inherently superior electrical characteristics and ability to house active electronics of silicon. This multi-modal device includes two sensing units. The first unit consists of a floating-gate field-effect transistor (FG-FET), which is used to monitor changes in pH in the sensing area. The threshold voltage of the FG-FET is regulated by a capacitively-coupled gate and by the changes in charge concentration in close proximity to the extension of the floating gate, which functions as the sensing electrode. The second unit uses the extension of the FG as microelectrode, in order to monitor the action potential of electrically active cells. The layout of the chip and its packaging are compatible with multi-electrode array measurement setups, which are commonly used in electrophysiology labs. The multi-functional sensing is demonstrated by monitoring the growth of induced pluripotent stem cell-derived cortical neurons. Our multi-modal sensor is a milestone in combined monitoring of different, physiologically-relevant parameters on the same device for future OoC platforms.

http://resolver.tudelft.nl/uuid:1ed0bc44-60d5-42d7-a0f6-769e528c99e6

https://doi.org/10.1038/s41598-023-34786-5

2045-2322

Scientific Reports, 13 (1), 8062

Institutional Repository

journal article

© 2023 H. Aydogmus, M. Hu, Lovro Ivancevic, Jean Philippe Frimat, Arn M.J.M. van den Maagdenberg, Pasqualina M Sarro, Massimo Mastrangeli