Print Email Facebook Twitter Multi-color electron microscopy by element-guided identification of cells, organelles and molecules Title Multi-color electron microscopy by element-guided identification of cells, organelles and molecules Author Scotuzzi, M. (TU Delft ImPhys/Charged Particle Optics) Kuipers, Jeroen (University Medical Center Groningen) Wensveen, D.I. (TU Delft ImPhys/Charged Particle Optics) De Boer, Pascal (University Medical Center Groningen) Hagen, C.W. (TU Delft ImPhys/Charged Particle Optics) Hoogenboom, J.P. (TU Delft ImPhys/Charged Particle Optics) Giepmans, Ben N.G. (University Medical Center Groningen) Date 2017-04-07 Abstract Cellular complexity is unraveled at nanometer resolution using electron microscopy (EM), but interpretation of macromolecular functionality is hampered by the difficulty in interpreting grey-scale images and the unidentified molecular content. We perform large-scale EM on mammalian tissue complemented with energy-dispersive X-ray analysis (EDX) to allow EM-data analysis based on elemental composition. Endogenous elements, labels (gold and cadmium-based nanoparticles) as well as stains are analyzed at ultrastructural resolution. This provides a wide palette of colors to paint the traditional grey-scale EM images for composition-based interpretation. Our proof-of-principle application of EM-EDX reveals that endocrine and exocrine vesicles exist in single cells in Islets of Langerhans. This highlights how elemental mapping reveals unbiased biomedical relevant information. Broad application of EM-EDX will further allow experimental analysis on large-scale tissue using endogenous elements, multiple stains, and multiple markers and thus brings nanometer-scale 'color-EM' as a promising tool to unravel molecular (de)regulation in biomedicine. To reference this document use: http://resolver.tudelft.nl/uuid:4da1eff4-cfcf-4484-8ff9-a001082ec912 DOI https://doi.org/10.1038/srep45970 ISSN 2045-2322 Source Scientific Reports, 7 Part of collection Institutional Repository Document type journal article Rights © 2017 M. Scotuzzi, Jeroen Kuipers, D.I. Wensveen, Pascal De Boer, C.W. Hagen, J.P. Hoogenboom, Ben N.G. Giepmans Files PDF srep45970.pdf 4.09 MB Close viewer /islandora/object/uuid:4da1eff4-cfcf-4484-8ff9-a001082ec912/datastream/OBJ/view