Print Email Facebook Twitter A DNA turbine powered by a transmembrane potential across a nanopore Title A DNA turbine powered by a transmembrane potential across a nanopore Author Shi, X. (TU Delft BN/Cees Dekker Lab; Katholieke Universiteit Leuven; Kavli institute of nanoscience Delft) Pumm, Anna Katharina (Technische Universität München) Maffeo, Christopher (University of Illinois at Urbana Champaign) Kohler, Fabian (Technische Universität München) Feigl, Elija (Technische Universität München) Zhao, W. (TU Delft RST/Storage of Electrochemical Energy; Kavli institute of nanoscience Delft) Verschueren, D.V. (TU Delft BN/Cees Dekker Lab; Kavli institute of nanoscience Delft) Golestanian, Ramin (Max Planck Institute for Dynamics and Self-Organisation; University of Oxford) Aksimentiev, Aleksei (University of Illinois at Urbana Champaign) Dietz, Hendrik (Technische Universität München) Dekker, C. (TU Delft BN/Cees Dekker Lab; Kavli institute of nanoscience Delft) Date 2023 Abstract Rotary motors play key roles in energy transduction, from macroscale windmills to nanoscale turbines such as ATP synthase in cells. Despite our abilities to construct engines at many scales, developing functional synthetic turbines at the nanoscale has remained challenging. Here, we experimentally demonstrate rationally designed nanoscale DNA origami turbines with three chiral blades. These DNA nanoturbines are 24–27 nm in height and diameter and can utilize transmembrane electrochemical potentials across nanopores to drive DNA bundles into sustained unidirectional rotations of up to 10 revolutions s−1. The rotation direction is set by the designed chirality of the turbine. All-atom molecular dynamics simulations show how hydrodynamic flows drive this turbine. At high salt concentrations, the rotation direction of turbines with the same chirality is reversed, which is explained by a change in the anisotropy of the electrophoretic mobility. Our artificial turbines operate autonomously in physiological conditions, converting energy from naturally abundant electrochemical potentials into mechanical work. The results open new possibilities for engineering active robotics at the nanoscale. To reference this document use: http://resolver.tudelft.nl/uuid:572005f8-f90c-407a-9fe2-93f38a4d60e5 DOI https://doi.org/10.1038/s41565-023-01527-8 ISSN 1748-3387 Source Nature Nanotechnology, 19 (3), 338-344 Part of collection Institutional Repository Document type journal article Rights © 2023 X. Shi, Anna Katharina Pumm, Christopher Maffeo, Fabian Kohler, Elija Feigl, W. Zhao, D.V. Verschueren, Ramin Golestanian, Aleksei Aksimentiev, Hendrik Dietz, C. Dekker Files PDF s41565_023_01527_8.pdf 4.95 MB Close viewer /islandora/object/uuid:572005f8-f90c-407a-9fe2-93f38a4d60e5/datastream/OBJ/view