A Bienzymatic Cascade for the Complete Hydrolysis of Phthalic Acid Esters

A Bienzymatic Cascade for the Complete Hydrolysis of Phthalic Acid Esters

Yang, Liu (South China University of Technology)
Ma, Yunjian (South China University of Technology; Macau University of Science and Technology)
Chen, Yebao (South China University of Technology)
Hollmann, F. (TU Delft BT/Biocatalysis)
Wang, Yonghua (GuangdongYoumeiInstituteof IntelligentBio-manufacturing, Guangdong)

2022

Phthalic acid esters (PAEs) are widely used as plastic additives to increase the flexibility and durability of plastics. Constantly leaching out from plastics, PAEs are ubiquitously found in the environment. As PAEs exhibit biological activities such as being endocrine disruptive, the quest for efficient degradation strategies continues. Here, we report a bienzymatic degradation system for PAEs to phthalic acid (PA) using a cascade comprising two hydrolases, EstJ6 and P8219. The reaction conditions were optimized with respect to concentrations of both enzymes, temperature, and initial pH. Finally, the substrate scope of the new cascade was investigated, revealing that particularly PAEs with relatively small alcohols were degraded to more than 90 %. This present study provides a potential doable biocatalytic strategy for the complete hydrolysis of PAEs.

cascade
degradation
hydrolase
phthalic acid
phthalic acid esters

http://resolver.tudelft.nl/uuid:80b324be-727f-4831-8a5e-6a93014fc4f0

https://doi.org/10.1002/slct.202201992

2023-02-10

2365-6549

ChemistrySelect, 7 (30)

Green Open Access added to TU Delft Institutional Repository ‘You share, we take care!’ – Taverne project https://www.openaccess.nl/en/you-share-we-take-care Otherwise as indicated in the copyright section: the publisher is the copyright holder of this work and the author uses the Dutch legislation to make this work public.

Institutional Repository

journal article

© 2022 Liu Yang, Yunjian Ma, Yebao Chen, F. Hollmann, Yonghua Wang