During the 1990s, Dutch bicycle policy took shape within the Bicycle Master Plan (BMP) framework, part of the Second Transport Structure Plan (Tweede Structuurschema Verkeer en Vervoer SVV-II a) for the period 1990-2010. A decentralized policy execution approach was followed, where the municipalities were encouraged to form their policy instruments related to bicycles and execution plans. The term "Cycling Climate" will be used in this research project about the cycling levels and bicycle use. The cycling climate success cannot be credited to the standalone policy but a combination of procedures such as transport, land-use, urban development, housing, environment, taxation, and parking sectors. These policies can be termed "Policy Mix ", contributing to the transition from motorized transport to sustainable active transport. Apart from the policy mixes, various other exogenous factors influence the cycling climate in municipalities. Studies have tried to evaluate the factors and policy instruments contributing to the uptake of cycling activities in The Netherlands. Still, scarce research or literature is available examining the combined effort of the policy mix in influencing cycling climate in the respective municipalities. Therefore, a comparative case study of Zwolle and Enschede municipalities is conducted to understand the impact of the policy mix approach on the cycling climate in the Netherlands. In Zwolle, the cycling climate has witnessed continuous improvements between 2000 and 2020. However, the policy mix during this period is characterized by weak consistency and credibility. The policy formulation process had remained coherent, whereas the policy implementation process had changed from being coherent to non-coherent during 2009-2020. These characteristics have remained the vital drivers of the policy mix success and seem to have influenced the cycling climate in the city. In Enschede, the cycling climate had declined during 2000 and 2010, before recovering and improving between 2010 and 2020. During the first decade, the policy mix did not exhibit any of the visible characteristics. However, during the second decade, the policy mix was characterized by credibility and consistent policy instruments. In addition, the process of policy formulation and implementation was coherent. These characteristics were the vital drivers of the policy mix success and could have influenced the rapid improvement in the cycling climate. The absence of a consistent and coherent policy mix coincided with the decline in cycling climate in Enschede during the period 2000-2008. The resurgence in cycling climate happened during the next decade when the municipality adopted a consistent, coherent, and credible policy mix. In Zwolle, a sustained and slightly improving cycling climate has coincided with the existence of a credible and weak-consistent policy mix. The consistency and coherency of the policy mix have changed in Zwolle over the two decades, but the policy mix has been supported by leadership and administrators. Hence, the policy mix design seems to influence the cycling climate and cycling policies in Zwolle and Enschede. The evidence from both the municipalities shows that following a policy mix approach has benefited the cycling climate within the municipalities. Furthermore, the political and administrative support and the participation of the interest groups have contributed to the success of the policy mix in both Zwolle and Enschede. Therefore, municipalities and policy researchers or consultants working towards improving the cycling climate can try the policy mix approach to improve the cycling climate within their respective municipalities.