Print Email Facebook Twitter Enantioselective Sulfoxidation of Thioanisole by Cascading a Choline Oxidase and a Peroxygenase in the Presence of Natural Deep Eutectic Solvents Title Enantioselective Sulfoxidation of Thioanisole by Cascading a Choline Oxidase and a Peroxygenase in the Presence of Natural Deep Eutectic Solvents Author Li, Yongru (South China University of Technology) Ma, Yunjian (South China University of Technology) Li, Peilin (South China University of Technology) Zhang, Xizhen (South China University of Technology) Ribitsch, Doris (acib GmbH) Alcalde, Miguel (Institute of Catalysis, CSIC, Madrid) Hollmann, F. (TU Delft BT/Biocatalysis) Wang, Yonghua (South China University of Technology) Date 2020 Abstract A bienzymatic cascade for selective sulfoxidation is presented. The evolved recombinant peroxygenase from Agrocybe aegeritra catalyses the enantioselective sulfoxidation of thioanisole whereas the choline oxidase from Arthrobacter nicotianae provides the H2 O2 necessary via reductive activation of ambient oxygen. The reactions are performed in choline chloride-based deep eutectic solvents serving as co-solvent and stoichiometric reductant at the same time. Very promising product concentrations (up to 15 mM enantiopure sulfoxide) and catalyst performances (turnover numbers of 150,000 and 2100 for the peroxygenase and oxidase, respectively) have been achieved. Subject biocatalysisenantioselectivitynatural deep eutectic solventsperoxygenasessulfoxidation To reference this document use: http://resolver.tudelft.nl/uuid:b9c412ce-4af0-49c7-9cfe-23892e133859 DOI https://doi.org/10.1002/cplu.201900751 ISSN 2192-6506 Source ChemPlusChem, 85 (1), 254-257 Part of collection Institutional Repository Document type journal article Rights © 2020 Yongru Li, Yunjian Ma, Peilin Li, Xizhen Zhang, Doris Ribitsch, Miguel Alcalde, F. Hollmann, Yonghua Wang Files PDF Li_et_al_2020_ChemPlusChem.pdf 684.62 KB Close viewer /islandora/object/uuid:b9c412ce-4af0-49c7-9cfe-23892e133859/datastream/OBJ/view