A multiwell plate Organ-on-Chip (OOC) device for in-vitro cell culture stimulation and monitoring

Title

A multiwell plate Organ-on-Chip (OOC) device for in-vitro cell culture stimulation and monitoring

 Author

Gaio, N. (TU Delft Electronic Components, Technology and Materials) ORCID 0000-0003-3332-1540
Waafi, A. (Student TU Delft)
Vlaming, M.L.H. (Ncardia)
Boschman, E. (Boschman Technologies)
Dijkstra, P. (Philips Innovation Services)
Nacken, O. (Ncardia)
Braam, S.R. (Ncardia)
Boucsein, C. (Multi Channel Systems GmbH)
Sarro, Pasqualina M (TU Delft Electronic Components, Technology and Materials) ORCID 0000-0002-2766-0880
Dekker, R. (TU Delft Electronic Components, Technology and Materials) ORCID 0000-0003-0839-4342

 Date

2018

 Abstract

This work presents the first multi-well plate that allows for simultaneous mechanical stimulation and electrical monitoring of multiple in-vitro cell cultures in parallel. Each well of the plate is equipped with an Organ-on-Chip (OOC) device consisting of a stretchable micro-electrode array (MEA). For the first time, a film assisted molding (FAM) process was employed to embed an OOC into a multi well plate format packaging. The functionality of the MEA in the device was assessed with electrochemical impedance spectroscopy. Moreover, the biocompatibility of the plate was demonstrated with cardiomyocytes derived from human induced pluripotent stem cells (iPSC) cultured in the wells.


 Subject

Electrodes
Monitoring
Silicon
Packaging
Biomedical monitoring
Biomembranes
Impedance

 To reference this document use:

http://resolver.tudelft.nl/uuid:c6d11c61-0a13-42a3-8655-e9dba97f287a

 DOI

https://doi.org/10.1109/MEMSYS.2018.8346549

 Publisher

IEEE, Piscataway, NJ

 Embargo date

2021-08-02

 ISBN

978-1-5386-4782-0

 Source

2018 IEEE Micro Electro Mechanical Systems, MEMS 2018, 2018-January

 Event

MEMS 2018, 2018-01-21 → 2018-01-25, Belfast, United Kingdom

 Bibliographical note

Green Open Access added to TU Delft Institutional Repository ‘You share, we take care!’ – Taverne project https://www.openaccess.nl/en/you-share-we-take-care Otherwise as indicated in the copyright section: the publisher is the copyright holder of this work and the author uses the Dutch legislation to make this work public.

 Part of collection

Institutional Repository

 Document type

conference paper

 Rights

© 2018 N. Gaio, A. Waafi, M.L.H. Vlaming, E. Boschman, P. Dijkstra, O. Nacken, S.R. Braam, C. Boucsein, Pasqualina M Sarro, R. Dekker
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