Print Email Facebook Twitter Plasma-Driven in Situ Production of Hydrogen Peroxide for Biocatalysis Title Plasma-Driven in Situ Production of Hydrogen Peroxide for Biocatalysis Author Yayci, Abdulkadir (Ruhr-Universität Bochum) Baraibar, Álvaro Gómez (Ruhr-Universität Bochum) Krewing, Marco (Ruhr-Universität Bochum) Fernandez Fueyo, E. (TU Delft BN/Greg Bokinsky Lab) Hollmann, F. (TU Delft BT/Biocatalysis) Alcalde, Miguel (Institute of Catalysis, CSIC, Madrid) Kourist, Robert (Ruhr-Universität Bochum; Graz University of Technology) Bandow, Julia E. (Ruhr-Universität Bochum) Date 2020 Abstract Peroxidases and peroxygenases are promising classes of enzymes for biocatalysis because of their ability to carry out one-electron oxidation reactions and stereoselective oxyfunctionalizations. However, industrial application is limited, as the major drawback is the sensitivity toward the required peroxide substrates. Herein, we report a novel biocatalysis approach to circumvent this shortcoming: in situ production of H2O2 by dielectric barrier discharge plasma. The discharge plasma can be controlled to produce hydrogen peroxide at desired rates, yielding desired concentrations. Using horseradish peroxidase, it is demonstrated that hydrogen peroxide produced by plasma treatment can drive the enzymatic oxidation of model substrates. Fungal peroxygenase is then employed to convert ethylbenzene to (R)-1-phenylethanol with an ee of >96 % using plasma-generated hydrogen peroxide. As direct treatment of the reaction solution with plasma results in reduced enzyme activity, the use of plasma-treated liquid and protection strategies are investigated to increase total turnover. Technical plasmas present a noninvasive means to drive peroxide-based biotransformations. Subject biocatalysisperoxidaseperoxidesperoxygenaseplasma chemistry To reference this document use: http://resolver.tudelft.nl/uuid:cc87f47c-d542-48cc-ac0b-72e244d70c4e DOI https://doi.org/10.1002/cssc.201903438 ISSN 1864-5631 Source ChemSusChem (Print): chemistry & sustainability, energy & materials, 13 (8), 2072-2079 Part of collection Institutional Repository Document type journal article Rights © 2020 Abdulkadir Yayci, Álvaro Gómez Baraibar, Marco Krewing, E. Fernandez Fueyo, F. Hollmann, Miguel Alcalde, Robert Kourist, Julia E. Bandow Files PDF cssc.201903438.pdf 1.43 MB Close viewer /islandora/object/uuid:cc87f47c-d542-48cc-ac0b-72e244d70c4e/datastream/OBJ/view