Print Email Facebook Twitter Opening of endothelial cell–cell contacts due to sonoporation Title Opening of endothelial cell–cell contacts due to sonoporation Author Beekers, D.I. (Erasmus MC) Vegter, Merel (Erasmus MC) Lattwein, Kirby R. (Erasmus MC) Mastik, Frits (Erasmus MC) Beurskens, Robert (Erasmus MC) van der Steen, A.F.W. (TU Delft ImPhys/Medical Imaging; Erasmus MC) de Jong, N. (TU Delft ImPhys/Medical Imaging; Erasmus MC) Verweij, M.D. (TU Delft ImPhys/Medical Imaging; Erasmus MC) Kooiman, Klazina (Erasmus MC) Date 2020 Abstract Ultrasound insonification of microbubbles can locally increase vascular permeability to enhance drug delivery. To control and optimize the therapeutic potential, we need to better understand the underlying biological mechanisms of the drug delivery pathways. The aim of this in vitro study was to elucidate the microbubble-endothelial cell interaction using the Brandaris 128 ultra-high-speed camera (up to 25 Mfps) coupled to a custom-built Nikon confocal microscope, to visualize both microbubble oscillation and the cellular response. Sonoporation and opening of cell-cell contacts by single αVβ3-targeted microbubbles (n = 152) was monitored up to 4 min after ultrasound insonification (2 MHz, 100–400 kPa, 10 cycles). Sonoporation occurred when microbubble excursion amplitudes exceeded 0.7 μm. Quantification of the influx of the fluorescent model drug propidium iodide upon sonoporation showed that the size of the created pore increased for larger microbubble excursion amplitudes. Microbubble-mediated opening of cell-cell contacts occurred as a cellular response upon sonoporation and did not correlate with the microbubble excursion amplitude itself. The initial integrity of the cell-cell contacts affected the susceptibly to drug delivery, since cell-cell contacts opened more often when cells were only partially attached to their neighbors (48%) than when fully attached (14%). The drug delivery outcomes were independent of nonlinear microbubble behavior, microbubble location, and cell size. In conclusion, by studying the microbubble–cell interaction at nanosecond and nanometer resolution the relationship between drug delivery pathways and their underlying mechanisms was further unraveled. These novel insights will aid the development of safe and efficient microbubble-mediated drug delivery. Subject Cell-cell contact openingDrug deliveryHigh-speed imagingMicrobubblesSonoporationUltrasound To reference this document use: http://resolver.tudelft.nl/uuid:ee49615f-4786-43c7-ad06-edd18463c0e1 DOI https://doi.org/10.1016/j.jconrel.2020.03.038 ISSN 0168-3659 Source Journal of Controlled Release: official journal of the Controlled Release Society, 322, 426-438 Part of collection Institutional Repository Document type journal article Rights © 2020 D.I. Beekers, Merel Vegter, Kirby R. Lattwein, Frits Mastik, Robert Beurskens, A.F.W. van der Steen, N. de Jong, M.D. Verweij, Klazina Kooiman Files PDF 1_s2.0_S0168365920301905_main.pdf 9.91 MB Close viewer /islandora/object/uuid:ee49615f-4786-43c7-ad06-edd18463c0e1/datastream/OBJ/view