Effect of temperature on the compositions of ladderane lipids in globally surveyed anammox populations

Effect of temperature on the compositions of ladderane lipids in globally surveyed anammox populations

Kouba, Vojtěch (University of Chemistry and Technology Prague)
Hůrková, Kamila (University of Chemistry and Technology Prague)
Navrátilová, Klára (University of Chemistry and Technology Prague)
Kok, Dana (University of Chemistry and Technology Prague)
Benáková, Andrea (University of Chemistry and Technology Prague)
Laureni, M. (TU Delft Sanitary Engineering)
van Niftrik, Laura (Radboud Universiteit Nijmegen)
van Loosdrecht, Mark C.M. (TU Delft BT/Environmental Biotechnology)
Weissbrodt, D.G. (TU Delft BT/Environmental Biotechnology)

2022

The adaptation of bacteria involved in anaerobic ammonium oxidation (anammox) to low temperatures will enable more efficient removal of nitrogen from sewage across seasons. At lower temperatures, bacteria typically tune the synthesis of their membrane lipids to promote membrane fluidity. However, such adaptation of anammox bacteria lipids, including unique ladderane phospholipids and especially shorter ladderanes with absent phosphatidyl headgroup, is yet to be described in detail. We investigated the membrane lipids composition (UPLC–HRMS/MS) and dominant anammox populations (16S rRNA gene amplicon sequencing, Fluorescence in situ hybridization) in 14 anammox enrichments cultivated at 10–37 °C. “Candidatus Brocadia” appeared to be the dominant organism in all but two laboratory enrichments of “Ca. Scalindua” and “Ca. Kuenenia”. At lower temperatures, the membranes of all anammox populations were composed of shorter [5]-ladderane ester (reduced chain length demonstrated by decreased fraction of C20/(C18 + C20)). This confirmed the previous preliminary evidence on the prominent role of this ladderane fatty acid in low-temperature adaptation. “Ca. Scalindua” and “Ca. Kuenenia” had distinct profile of ladderane lipids compared to “Ca. Brocadia” biomasses with potential implications for adaptability to low temperatures. “Ca. Brocadia” membranes contained a much lower amount of C18 [5]-ladderane esters than reported in the literature for “Ca. Scalindua” at similar temperature and measured here, suggesting that this could be one of the reasons for the dominance of “Ca. Scalindua” in cold marine environments. Furthermore, we propose additional and yet unreported mechanisms for low-temperature adaptation of anammox bacteria, one of which involves ladderanes with absent phosphatidyl headgroup. In sum, we deepen the understanding of cold anammox physiology by providing for the first time a consistent comparison of anammox-based communities across multiple environments.

Anaerobic ammonium oxidation
Candidatus Brocadia
Candidatus Scalindua
Effect of temperature
Ladderane phospholipids

http://resolver.tudelft.nl/uuid:f03aacc3-655f-49d6-9fb2-405b610e7a58

https://doi.org/10.1016/j.scitotenv.2022.154715

2022-09-22

0048-9697

Science of the Total Environment, 830

Green Open Access added to TU Delft Institutional Repository ‘You share, we take care!’ – Taverne project https://www.openaccess.nl/en/you-share-we-take-care Otherwise as indicated in the copyright section: the publisher is the copyright holder of this work and the author uses the Dutch legislation to make this work public.

Institutional Repository

journal article

© 2022 Vojtěch Kouba, Kamila Hůrková, Klára Navrátilová, Dana Kok, Andrea Benáková, M. Laureni, Laura van Niftrik, Mark C.M. van Loosdrecht, D.G. Weissbrodt, More Authors