Print Email Facebook Twitter Morphological and Surface Potential Characterization of Protein Nanobiofilm Formation on Magnesium Alloy Oxide Title Morphological and Surface Potential Characterization of Protein Nanobiofilm Formation on Magnesium Alloy Oxide: Their Role in Biodegradation Author Rahimi, E. (TU Delft Materials Science and Engineering; Università degli Studi di Udine) Imani, Amin (University of British Columbia) Lekka, Maria (Basque Research and Technology Alliance (BRTA)) Andreatta, Francesco (Università degli Studi di Udine) Gonzalez Garcia, Y. (TU Delft Team Yaiza Gonzalez Garcia) Mol, J.M.C. (TU Delft Team Arjan Mol) Asselin, Edouard (University of British Columbia) Fedrizzi, Lorenzo (Università degli Studi di Udine) Department Materials Science and Engineering Date 2022 Abstract The formation of a protein nanobiofilm on the surface of degradable biomaterials such as magnesium (Mg) and its alloys influences metal ion release, cell adhesion/spreading, and biocompatibility. During the early stage of human body implantation, competition and interaction between inorganic species and protein molecules result in a complex film containing Mg oxide and a protein layer. This film affects the electrochemical properties of the metal surface, the protein conformational arrangement, and the electronic properties of the protein/Mg oxide interface. In this study, we discuss the impact of various simulated body fluids, including sodium chloride (NaCl), phosphate-buffered saline (PBS), and Hanks' solutions on protein adsorption, electrochemical interactions, and electrical surface potential (ESP) distribution at the adsorbed protein/Mg oxide interface. After 10 min of immersion in NaCl, atomic force microscopy (AFM) and scanning Kelvin probe force microscopy (SKPFM) showed a higher surface roughness related to enhanced degradation and lower ESP distribution on a Mg-based alloy than those in other solutions. Furthermore, adding bovine serum albumin (BSA) to all solutions caused a decline in the total surface roughness and ESP magnitude on the Mg alloy surface, particularly in the NaCl electrolyte. Using SKPFM surface analysis, we detected a protein nanobiofilm (∼10-20 nm) with an aggregated and/or fibrillary morphology only on the Mg surface exposed in Hanks' and PBS solutions; these surfaces had a lower ESP value than the oxide layer. Subject adsorptionalloyselectrochemistryoxidessurface potential To reference this document use: http://resolver.tudelft.nl/uuid:fd0f2019-7f58-4193-ad58-ba74aef7901f DOI https://doi.org/10.1021/acs.langmuir.2c01540 ISSN 0743-7463 Source Langmuir: the ACS journal of surfaces and colloids, 38 (35), 10854-10866 Part of collection Institutional Repository Document type journal article Rights © 2022 E. Rahimi, Amin Imani, Maria Lekka, Francesco Andreatta, Y. Gonzalez Garcia, J.M.C. Mol, Edouard Asselin, Lorenzo Fedrizzi Files PDF acs.langmuir.2c01540.pdf 3.42 MB Close viewer /islandora/object/uuid:fd0f2019-7f58-4193-ad58-ba74aef7901f/datastream/OBJ/view