Live-cell imaging reveals the trade-off between target search flexibility and efficiency for Cas9 and Cas12a

CRISPR-Cas systems have widely been adopted as genome editing tools, with two frequently employed Cas nucleases being SpyCas9 and LbCas12a. Although both nucleases use RNA guides to find and cleave target DNA sites, the two enzymes differ in terms of protospacer-adjacent motif (PAM) requirements, guide architecture and cleavage mechanism. In...

Mapping Wuhan: Morphological atlas of the Urbanization of a Chinese City

Chinese cities have been expanding since the early 1980s under trends of rapid modernization, urbanization and globalization. Since then they have changed dramatically, and have in the process lost many of their traditional environments and spatial characteristics. Urban planners and designers have been and are facing unprecedented challenges in...

Re-printing architectural heritage: Exploring current 3D printing and scanning technologies

Additive Manufacturing (commonly known as 3D printing) technology has become a global phenomenon. In the domain of heritage, 3D printing is seen as a time and cost efficient method for restoring vulnerable architectural structures. The technology can also provide an opportunity to reproduce missing or destroyed cultural heritage, in the cases...

The Delft Fundamentals: integration of disciplines, projects and analysis

As part of the renewal in 2013/2014 of the bachelor education curriculum in the Faculty of Architecture and the Built Environment, Delft University of Technology, a new study programme was prepared on the Fundamentals (in Dutch: Grondslagen) of spatial design. The teaching approach, visually presented by some examples and explained in this...

Voetlicht op het stedebouwkundig ontwerpen in het fin de siècle: Naar een stedebouw van conventie

Stedelijke transformaties: Actuele opgaven in de stad en de rol van de stedebouwkundige discipline; samenvattende bevindingen van stedebouwkundig onderzoek 1992-1997

Stedelijke transformaties: Actuele opgaven in de stad en de rol van de stedebouwkundige discipline

Het onderzoekprogramma 1992-1997 van de (inmiddels voormalige) vakgroep Stedebouwkunde, Faculteit der Bouwkunde, Technische Universiteit Delft heeft twee onderzoekvelden omvat: Metropoolvorming en Stedelijke transformaties. Dit boek geeft een overzicht van de conclusies van de deelprojecten van het onderzoek naar de stedelijke transformaties.