- document
-
Hung, S. (author), Llobet Rosell, Arnau (author), Jurriens, Daphne (author), Soloviev, O.A. (author), Kapitein, Lukas C. (author), Grußmayer, K.S. (author), Neukomm, Lukas J. (author), Verhaegen, M.H.G. (author), Smith, C.S. (author)Single-molecule localization microscopy (SMLM) enables the high-resolution visualization of organelle structures and the precise localization of individual proteins. However, the expected resolution is not achieved in tissue as the imaging conditions deteriorate. Sample-induced aberrations distort the point spread function (PSF), and high...journal article 2022
- document
-
Hung, S. (author), Cnossen, J.P. (author), Fan, D. (author), Siemons, Marijn (author), Jurriens, Daphne (author), Grußmayer, K.S. (author), Soloviev, O.A. (author), Kapitein, Lukas C. (author), Smith, C.S. (author)High-NA light sheet illumination can improve the resolution of single-molecule localization microscopy (SMLM) by reducing the background fluorescence. These approaches currently require custom-made sample holders or additional specialized objectives, which makes the sample mounting or the optical system complex and therefore reduces the...journal article 2022