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Oyibo, P.O. (author), Agbana, T.E. (author), van Lieshout, Lisette (author), Oyibo, Wellington (author), Diehl, J.C. (author), Vdovin, Gleb (author)Traditionally, automated slide scanning involves capturing a rectangular grid of field-of-view (FoV) images which can be stitched together to create whole slide images, while the autofocusing algorithm captures a focal stack of images<br/>to determine the best in-focus image. However, these methods can be timeconsuming due to the need for X-, Y-...journal article 2024
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Makau-Barasa, Louise (author), Assefa, Liya (author), Solomon, Jacob (author), A-Enegela, Juliana (author), Damen, James G. (author), Popoola, Samuel (author), Diehl, J.C. (author), Vdovin, Gleb (author), Agbana, T.E. (author)In this research, we report on the performance of automated optical digital detection and quantification of Schistosoma haematobium provided by AiDx NTDx multi-diagnostic Assist microscope. Our study was community-based, and a convenient sampling method was used in 17 communities in Abuja Nigeria, based on the disease prevalence information...journal article 2023
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Oyibo, P.O. (author), Meulah, Brice (author), Bengtson, Michel (author), Lieshout, Lisette van (author), Oyibo, Wellington (author), Diehl, J.C. (author), Vdovin, Gleb (author), Agbana, T.E. (author)Purpose: Automated diagnosis of urogenital schistosomiasis using digital microscopy images of urine slides is an essential step toward the elimination of schistosomiasis as a disease of public health concern in Sub-Saharan African countries. We create a robust image dataset of urine samples obtained from field settings and develop a two-stage...journal article 2023
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Oyibo, P.O. (author), Jujjavarapu, S. (author), Meulah, Brice (author), Agbana, T.E. (author), Braakman, I.G. (author), van Diepen, Angela (author), Bengtson, M.L. (author), van Lieshout, Lisette (author), Andi, Wellington Oyibo (author), Vdovin, Gleb (author), Diehl, J.C. (author)For many parasitic diseases, the microscopic examination of clinical samples such as urine and stool still serves as the diagnostic reference standard, primarily because microscopes are accessible and cost-effective. However, conventional microscopy is laborious, requires highly skilled personnel, and is highly subjective. Requirements for...journal article 2022
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Makau-Barasa, Louise (author), Aderogba, Moses O. (author), Bell, David (author), Popoola, Samuel (author), Agbana, T.E. (author), Diehl, J.C. (author), Vdovin, Gleb (author)To achieve the WHO’s goal of sustained elimination of Lymphatic Filariasis disease, there is the need for accurate diagnostic device especially in areas of low-intensity infections. This project, which is a collaboration of the Nigerian Federal Ministry of Health (FMoH), AiDx and funded by THE END FUND is exploring the performance capacity of...report 2022
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Soloviev, O.A. (author), Noom, J. (author), Nguyen, Hieu Thao (author), Vdovin, Gleb (author), Verhaegen, M.H.G. (author)We investigate the general adjustment of projection-based phase retrieval algorithms for use with saturated data. In the phase retrieval problem, model fidelity of experimental data containing a non-zero background level, fixed pattern noise, or overexposure, often presents a serious obstacle for standard algorithms. Recently, it was shown...conference paper 2022
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de Bruijne, Bas (author), Vdovin, Gleb (author), Soloviev, O.A. (author)We have applied a combination of blind deconvolution and deep learning to the processing of Shack-Hartmann images.By using the intensity information contained in spot positions, and the fine structure of the separate images created by the lenslets,we have increased the sensitivity and resolution of the sensor over the limit defined by...journal article 2022
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van Grootheest, D.N. (author), Agbana, T.E. (author), Diehl, J.C. (author), van Diepen, Angela (author), Bezzubik, Vitalii (author), Vdovin, Gleb (author)Significance: Particle field holography is a versatile technique to determine the size and distribution of moving or stationary particles in air or in a liquid without significant disturbance of the sample volume. Although this technique is applied in biological sample analysis, it is limited to small sample volumes, thus increasing the number...journal article 2021
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Agbana, T.E. (author), Nijman, Patrick (author), Hoeboer, M.D.B. (author), van Grootheest, D.N. (author), van Diepen, Angela (author), van Lieshout, Lisette (author), Diehl, J.C. (author), Verhaegen, M.H.G. (author), Vdovin, Gleb (author)We present a simple method for the diagnosis of urinary schistosomiasis using an in-line lensless holographic microscope combined with flow cytometry technique. Using simple image processing algorithms and binary image classifier, our system provides automated detection of Schistosoma haematobium eggs in infected urine samples. Registered...conference paper 2020
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Heemels, Alex (author), Agbana, T.E. (author), Pereira, S.F. (author), Diehl, J.C. (author), Verhaegen, M.H.G. (author), Vdovin, Gleb (author)Fourier Ptychography is a computational imaging technique able to decouple high resolution from wide field of view, bypassing the diffraction limit of the microscope. Since it does not rely on high precision mechanics or fluorescent imaging, it is of practical interest for implementation in low scale devices. Despite its gains, realizing a...conference paper 2020
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Pozzi, P. (author), Smith, C.S. (author), Carroll, E.C.M. (author), Wilding, D. (author), Soloviev, O.A. (author), Booth, Martin (author), Vdovin, Gleb (author), Verhaegen, M.H.G. (author)Inhomogeneities in the refractive index of a biological microscopy sample can introduce phase aberrations, severely impairing the quality of images. Adaptive optics can be employed to correct for phase aberrations and improve image quality. However, conventional adaptive optics can only correct a single phase aberration for the whole field of...journal article 2020
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Pozzi, P. (author), Maddalena, L. (author), Ceffa, N.G. (author), Soloviev, O.A. (author), Vdovin, Gleb (author), Carroll, E.C.M. (author), Verhaegen, M.H.G. (author)The use of spatial light modulators to project computer generated holograms is a common strategy for optogenetic stimulation of multiple structures of interest within a three-dimensional volume. A common requirement when addressing multiple targets sparsely distributed in three dimensions is the generation of a points cloud, focusing excitation...journal article 2019
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Wilding, D. (author), Pozzi, P. (author), Soloviev, O.A. (author), Vdovin, Gleb (author), Verhaegen, M.H.G. (author)In life sciences, interest in the microscopic imaging of increasingly complex three dimensional samples, such as cell spheroids, zebrafish embryos, and in vivo applications in small animals, is growing quickly. Due to the increasing complexity of samples, more and more life scientists are considering the implementation of adaptive optics in...conference paper 2018
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Pozzi, P. (author), Wilding, D. (author), Soloviev, O.A. (author), Vdovin, Gleb (author), Verhaegen, M.H.G. (author)In this work, we present a new confocal laser scanning microscope capable to perform sensorless wavefront optimization in real time. The device is a parallelized laser scanning microscope in which the excitation light is structured in a lattice of spots by a spatial light modulator, while a deformable mirror provides aberration correction and...conference paper 2018
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Wilding, D. (author), Pozzi, P. (author), Soloviev, O.A. (author), Vdovin, Gleb (author), Verhaegen, M.H.G. (author)Three-dimensional microscopy suffers from sample-induced aberrations that reduce the resolution and lead to misinterpretations of the object distribution. In this paper, the resolution of a three-dimensional fluorescent microscope is significantly improved by introducing an amplitude diversity in the form of a binary amplitude mask positioned...journal article 2018
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Kazasidis, Orestis (author), Verpoort, Sven (author), Soloviev, O.A. (author), Vdovin, Gleb (author), Verhaegen, M.H.G. (author), Wittrock, Ulrich (author)With a view to the next generation of large space telescopes, we investigate guide-starfree, image-based aberration correction using a unimorph deformable mirror in a plane conjugate to the primary mirror.We designed and built a high-resolution imaging testbed to evaluate control algorithms. In this paper we use an algorithm based on the...journal article 2018
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Agbana, T.E. (author), Diehl, J.C. (author), van Pul, Fiona (author), Khan, Shahid M. (author), Patlan, Vsevolod (author), Verhaegen, M.H.G. (author), Vdovin, Gleb (author)We have optimized the design and imaging procedures, to clearly resolve the malaria parasite in Giemsa-stained thin blood smears, using simple low-cost cellphone-based microscopy with oil immersion. The microscope uses a glass ball as the objective and the phone camera as the tube lens. Our optimization includes the optimal choice of the ball...journal article 2018
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Pozzi, P. (author), Soloviev, O.A. (author), Wilding, D. (author), Vdovin, Gleb (author), Verhaegen, M.H.G. (author)We report on a universal sample-independent sensorless adaptive optics method, based on modal optimization of the second moment of the fluorescence emission from a point-like excitation. Our method employs a sample-independent precalibration, performed only once for the particular system, to establish the direct relation between the image...journal article 2018
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Wilding, D. (author), Pozzi, P. (author), Soloviev, O.A. (author), Vdovin, Gleb (author), Fiolka, Reto (author), Verhaegen, M.H.G. (author)The light-sheet fluorescence microscopy is an excellent tool for the investigation of large three dimensional microscopy samples at the cellular level, however, the ability to resolve features is strongly affected by the presence of scattering and aberrations. These effects are two fold in light-sheet microscopy, as the illumination path...conference paper 2018
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Taballione, Caterina (author), Agbana, T.E. (author), Vdovin, Gleb (author), Hoekman, Marcel (author), Wevers, Lennart (author), Kalkman, J. (author), Verhaegen, M.H.G. (author), van der Slot, Peter J.M. (author), Boller, Klaus-Jochen (author)Wavelength meters are central for many applications such as in telecommunication systems or laser monitoring. The primary function of a wavelength meter is to provide an output signal that changes sensitively with the wavelength of the input light. Of central importance is the reproducibility of the output signal even in the presence of external...poster 2017