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Kulshreshtha, Y. (author), Vardon, P.J. (author), Du, Y. (author), Habert, G. (author), Vissac, A. (author), Morel, J.C. (author), van Loosdrecht, Mark C.M. (author), Mota, Nelson (author), Jonkers, H.M. (author)Earthen construction is re-gaining popularity as an ecological and economical alternative to contemporary building materials. While building with earth offers several benefits, its performance due to water ingress is a concern for its widespread application. This limitation is often solved by adding chemical stabilisers such as Portland cement...journal article 2022
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Soler Jofra, A. (author), Schmidtchen, Lisbeth (author), Olmo, Lluc (author), van Loosdrecht, Mark C.M. (author), Pérez, Julio (author)Hydroxylamine is a nitrogen intermediate of ammonium oxidizing bacteria (AOB) that can transiently accumulate during nitrification. The impact of hydroxylamine on aerobic ammonium oxidations is still obscure. In the present study the short and long term impact of hydroxylamine on partial nitritation granular sludge was investigated. Dissolved...journal article 2022
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Wijdeveld, W. K. (author), Prot, T.J.F. (author), Sudintas, G. (author), Kuntke, P. (author), Korving, L. (author), van Loosdrecht, Mark C.M. (author)Phosphorus (P) is an essential resource for food production and chemical industry. Phosphorus use has to become more sustainable and should include phosphorus recycling from secondary sources. About 20% of P ends up in sewage sludge, making this a substantial secondary P source. There is currently a technological gap to recover P from sludge...journal article 2022
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Kouba, Vojtěch (author), Hůrková, Kamila (author), Navrátilová, Klára (author), Kok, Dana (author), Benáková, Andrea (author), Laureni, M. (author), van Niftrik, Laura (author), van Loosdrecht, Mark C.M. (author), Weissbrodt, D.G. (author)The adaptation of bacteria involved in anaerobic ammonium oxidation (anammox) to low temperatures will enable more efficient removal of nitrogen from sewage across seasons. At lower temperatures, bacteria typically tune the synthesis of their membrane lipids to promote membrane fluidity. However, such adaptation of anammox bacteria lipids,...journal article 2022
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Chen, Xingyu (author), Lee, Yu Jen (author), Yuan, Tian (author), Lei, Zhongfang (author), Adachi, Yasuhisa (author), Zhang, Zhenya (author), Lin, Y. (author), van Loosdrecht, Mark C.M. (author)A reasonable recovery of excess sludge may shift the waste into wealth. Recently an increasing attention has been paid to the recycling of extracellular biopolymers from conventional and advanced biological wastewater treatment systems such as flocculent activated sludge (AS), bacterial aerobic granular sludge (AGS), and algal-bacterial AGS...review 2022
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Seviour, R (author), Tooker, Nick (author), Vainio, Pirjo (author), Dueholm, Morten Kam Dahl (author), Nierychlo, Marta (author), Andersen, Kasper Skytte (author), van Loosdrecht, Mark C.M. (author), Weissbrodt, D.G. (author), Rossetti, S (author)Microbial communities are responsible for biological wastewater treatment, but our knowledge of their diversity and function is still poor. Here, we sequence more than 5 million high-quality, full-length 16S rRNA gene sequences from 740 wastewater treatment plants (WWTPs) across the world and use the sequences to construct the ‘MiDAS 4’...journal article 2022
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Estevez Alonso, A. (author), Arias-Buendía, María (author), Pei, R. (author), van Veelen, H. Pieter J. (author), van Loosdrecht, Mark C.M. (author), Kleerebezem, R. (author), Werker, A. (author)Activated sludge from municipal wastewater treatment processes can be used directly for the production of biodegradable polyesters from the family of polyhydroxyalkanoates (PHAs). However, municipal activated sludge typically cannot accumulate PHAs to very high levels and often low yields of polymer produced on substrate are observed. In the...journal article 2022
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Estevez Alonso, A. (author), Altamira-Algarra, Beatriz (author), Arnau-Segarra, César (author), van Loosdrecht, Mark C.M. (author), Kleerebezem, R. (author), Werker, A. (author)The developments of mixed culture polyhydroxyalkanoate production has been directed to maximize the biomass PHA content with limited attention to polymer quality. Direct comparison of PHA accumulation literature is challenging, and even regularly contradicting in reported results, due to underlying differences that are not well expressed. A...journal article 2022
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Tomas Martinez, S. (author), Chen, Le Min (author), Pabst, Martin (author), Weissbrodt, D.G. (author), van Loosdrecht, Mark C.M. (author), Lin, Y. (author)Abstract: Pseudaminic and legionaminic acids are a subgroup of nonulosonic acids (NulOs) unique to bacterial species. There is a lack of advances in the study of these NulOs due to their complex synthesis and production. Recently, it was seen that “Candidatus Accumulibacter” can produce Pse or Leg analogues as part of its extracellular...journal article 2022
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Sharma, Keshab (author), Gutierrez, Oriol (author), Yuan, Zhiguo (author), Daelman, Matthijs R.J. (author), van Loosdrecht, Mark C.M. (author), Volcke, Eveline I.P. (author)This chapter provides a review of the models available for estimating the production and emission of methane from wastewater collection and treatment systems. The details of a number of mechanistic models as well as the simplified empirical models have been summarized. Their limitations have been identified and general methods for calibration...book chapter 2022
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Kosar, Sadiye (author), Isik, Onur (author), Cicekalan, Busra (author), Gulhan, Hazal (author), Sagir Kurt, Ece (author), Atli, Ezgi (author), Basa, Safak (author), Ozgun, Hale (author), van Loosdrecht, Mark C.M. (author)Aerobic granules contain microorganisms that are responsible for carbon, nitrogen, and phosphorus removal in aerobic granular sludge (AGS) process in which aerobic/anoxic/anaerobic layers (from surface to core) occur in a single granule. Optimizing the aerobic granular sludge (AGS) process for granulation and efficient nutrient removal can be...journal article 2022
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Olavarria, Karel (author), Pijman, Yared O. (author), Cabrera, Ricardo (author), van Loosdrecht, Mark C.M. (author), Wahl, S.A. (author)The coupling of PHB generation with NADH reoxidation is required to generate PHB as a fermentation product. A fundamental trait to accomplish this feature is to express a functional NADH-preferring acetoacetyl-CoA reductase, engaged in PHB accumulation. One way to obtain such a reductase is by engineering the cofactor preference of the...journal article 2022
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Pei, R. (author), Vicente-Venegas, Gerard (author), van Loosdrecht, Mark C.M. (author), Kleerebezem, R. (author), Werker, A. (author)Polyhydroxyalkanoate accumulation experiments at pilot scale were performed with fullscale municipal waste activated sludge. Development of biomass PHA content was quantified by thermogravimetric analysis. Over 48 h the biomass reached up to 0.49 ± 0.03 gPHA/gVSS (n=4). Samples were processed in parallel to characterise the distribution of...journal article 2022
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Hao, Xiaodi (author), Wang, Xiangyang (author), Shi, Chen (author), van Loosdrecht, Mark C.M. (author), Wu, Yuanyuan (author)Sludge incineration and seawater desalination are two approaches that can be used in the disposal of waste activated sludge (WAS) and for obtaining fresh water. As resource recovery from wastewater treatment and water purification is a topic of particular interest in these times, “water mining” has become a focus of research, with phosphate/P...journal article 2022
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Kouba, Vojtěch (author), Hůrková, Kamila (author), Navrátilová, Klára (author), Kok, Dana (author), Benáková, Andrea (author), Laureni, M. (author), Vodičková, Patricie (author), van Loosdrecht, Mark C.M. (author), Weissbrodt, D.G. (author)The application of partial nitritation-anammox (PN/A) under mainstream conditions can enable substantial cost savings at wastewater treatment plants (WWTPs), but how process conditions and cell physiology affect anammox performance at psychrophilic temperatures below 15 °C remains poorly understood. We tested 14 anammox communities, including...journal article 2022
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Ekholm, Jennifer (author), Persson, Frank (author), de Blois, Mark (author), Modin, Oskar (author), Pronk, M. (author), van Loosdrecht, Mark C.M. (author), Suarez, Carolina (author), Gustavsson, David J.I. (author), Wilén, Britt Marie (author)Aerobic granular sludge (AGS) plants have gained growing interest and application due to their low energy demand, small footprint, and low operational costs. However, the fulfilment of strict discharge limits for nitrogen and phosphorus, vast seasonal temperature variations, and large peaks in influent flows may pose challenges to the...journal article 2022
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Prot, T.J.F. (author), Korving, L. (author), van Loosdrecht, Mark C.M. (author)In a wastewater treatment plant (WWTP), several sludge streams exist and the composition of their liquid phase varies with time and place. For evaluating the potential for formation of precipitates and equilibria for weak acids/bases, the ionic strength and chemical composition need to be known. This information is often not available in...journal article 2022
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Palmeros Parada, M.D.M. (author), van der Wielen, L.A.M. (author), Osseweijer, P. (author), van Loosdrecht, Mark C.M. (author), Pashaei Kamali, F. (author), Posada Duque, J.A. (author), van der Putten, Wim H. (author)Aviation biofuels are promising to reduce carbon emissions in the aviation sector. However, emerging concerns over biofuels indicate a need for sustainability analyses that take into consideration the context around biofuel production. Here, we present a novel ex-ante sustainability analysis of production alternatives for aviation biofuel in...book chapter 2022
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Pei, R. (author), Estevez Alonso, A. (author), Ortiz-Seco, Laura (author), van Loosdrecht, Mark C.M. (author), Kleerebezem, R. (author), Werker, A. (author)Municipal activated sludge can be used for polyhydroxyalkanoate (PHA) production, when supplied with volatile fatty acids. In this work, standardized PHA accumulation assays were performed with different activated sludge to determine (1) the maximum biomass PHA content, (2) the degree of enrichment (or volume-to-volume ratio of PHA...journal article 2022
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Goedhart, R.C. (author), Müller, S. (author), van Loosdrecht, Mark C.M. (author), van Halem, D. (author)Iron in anaerobic groundwater is commonly removed by oxidation followed by sand filtration. This produces large volumes of iron(III)(hydr)oxide sludge with little value. Our research investigates the novel concept of anaerobic iron(II) recovery from groundwater as the valuable mineral vivianite (Fe<sub>3</sub>(PO<sub>4</sub>)<sub>2</sub> • 8...journal article 2022