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van Dijk, Raymond (author), Kalisvaart, D. (author), Cnossen, J.P. (author), Smith, C.S. (author)Single-molecule localization microscopy requires sparse activation of emitters to circumvent the diffraction limit. In densely labeled or thick samples, overlap of emitter images is inevitable. Single-molecule localization of these samples results in a biased parameter estimate with a wrong model of the number of emitters. On the other hand,...journal article 2023
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Kalisvaart, D. (author), Cnossen, J.P. (author), Hung, S. (author), Stallinga, S. (author), Verhaegen, M.H.G. (author), Smith, C.S. (author)Modulation enhanced single-molecule localization microscopy (meSMLM) methods improve the localization precision by using patterned illumination to encode additional position information. Iterative meSMLM (imeSMLM) methods iteratively generate prior information on emitter positions, used to locally improve the localization precision during...journal article 2022
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Houwink, Quint (author), Kalisvaart, D. (author), Hung, S. (author), Cnossen, J.P. (author), Fan, D. (author), Mos, Paul (author), Ülkü, Arin Can (author), Bruschini, Claudio (author), Charbon-Iwasaki-Charbon, E. (author), Smith, C.S. (author)Single-photon avalanche diode (SPAD) arrays can be used for single-molecule localization microscopy (SMLM) because of their high frame rate and lack of readout noise. SPAD arrays have a binary frame output, which means photon arrivals should be described as a binomial process rather than a Poissonian process. Consequentially, the theoretical...journal article 2021