Boltje, D.B. (author), Hoogenboom, J.P. (author), Jakobi, A. (author), Jensen, Grant J. (author), Jonker, C.T.H. (author), Kaag, Max J. (author), de Agrela Pinto, C. (author), van der Wee, E.B. (author), Hoedt, Sander Den (author) Cryogenic electron tomography (cryo-ET) combined with sub-tomogram averaging, allows in-situ visualization and structure determination of macromolecular complexes at sub-nanometre resolution. Cryogenic focused ion beam (cryo-FIB) micromachining is used to prepare a thin lamella-shaped sample out of a frozen-hydrated cell for cryo-ET imaging,...
journal article 2022
