Microscopy-based single-cell proteomic profiling reveals heterogeneity in DNA damage response dynamics

Single-cell proteomics has the potential to decipher tumor heterogeneity, and a method like single-cell proteomics by mass spectrometry (SCoPE-MS) allows profiling several tens of single cells for >1,000 proteins per cell. This method, however, cannot link the proteome of individual cells with phenotypes of interest. Here, we developed a...

HoloLearn: Using holograms to support naturalistic interaction in virtual classrooms

Traditional online communications tools used in education are limited in terms of fostering naturalistic or life-like interaction. Such limited interactions in classrooms can negatively impact learning. Holograms are promising tools that show potential to overcome such limitations by affording more life-like interactions in virtual classrooms...

Iterative tuning of feedforward IPC for two-bladed wind turbines

At present, the cost of offshore wind energy does not meet the level of onshore wind and fossil-based energy sources. One way to extend the turbine lifetime, and thus reduce cost, is by reduction of the fatigue loads of blades and other turbine parts using Individual Pitch Control (IPC). This type of control, which is generally implemented by...

A comparison between numerical predictions and theoretical and experimental results for laminar core-annular flow

high-viscosity liquid core surrounded by a laminar low-viscosity liquid annular layer through a vertical pipe. The numerical results are compared with theoretical results from linear stability calculations and with experimental data. The comparison is good and the general conclusion of our study is that it is very well possible to simulate...

On the levitation force in horizontal core-annular flow with a large viscosity ratio and small density ratio

A numerical study has been made of horizontal core-annular flow: the flow of a high-viscosity liquid core surrounded by a low-viscosity liquid annular layer through a horizontal pipe. Special attention is paid to the question how the buoyancy force on the core, caused by a density difference between the core and the annular layer, is...

Towards a Flexible ICT-Architecture for Multi-Channel E-Government Service Provisioning

The planning and subsequent nationwide implementation of E-government service provisioning faces a number of challenges at the level of municipalities in the Netherlands. Initiatives are confronted with a highly fragmented ICT-architecture that has been vertically organized around departments and with hardly any common horizontal functionality....