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de Hulster, A.F. (author), Mooiman, C. (author), Timmermans, Rowin (author), Mans, R. (author)Evolutionary engineering of microbes provides a powerful tool for untargeted optimization of (engineered) cell factories and identification of genetic targets for further research. Directed evolution is an intrinsically time-intensive effort, and automated methods can significantly reduce manual labor. Here, design considerations for various...journal article 2022
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Dekker, W.J.C. (author), Jürgens, H. (author), Ortiz Merino, R.A. (author), Mooiman, C. (author), van den Berg, Remon (author), Kaljouw, Astrid (author), Mans, R. (author), Pronk, J.T. (author)While thermotolerance is an attractive trait for yeasts used in industrial ethanol production, oxygen requirements of known thermotolerant species are incompatible with process requirements. Analysis of oxygen-sufficient and oxygen-limited chemostat cultures of the facultatively fermentative, thermotolerant species Ogataea parapolymorpha...journal article 2022
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Postma, E.D. (author), Else-Hassing, J. (author), Mangkusaputra, Venda (author), Geelhoed, J. (author), de la Torre, P. (author), van den Broek, M.A. (author), Mooiman, C. (author), Pabst, Martin (author), Daran, J.G. (author), Daran-Lapujade, P.A.S. (author)The construction of powerful cell factories requires intensive genetic engineering for the addition of new functionalities and the remodeling of native pathways and processes. The present study demonstrates the feasibility of extensive genome reprogramming using modular, specialized de novo-assembled neochromosomes in yeast. The in vivo...journal article 2022
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Perli, T. (author), Vos, A.M. (author), Bouwknegt, J. (author), Dekker, W.J.C. (author), Wiersma, S.J. (author), Mooiman, C. (author), Ortiz Merino, R.A. (author), Daran, J.G. (author), Pronk, J.T. (author)Neocallimastigomycetes are unique examples of strictly anaerobic eukaryotes. This study investigates how these anaerobic fungi bypass reactions involved in synthesis of pyridine nucleotide cofactors and coenzyme A that, in canonical fungal pathways, require molecular oxygen. Analysis of Neocallimastigomycetes proteomes identified a candidate...journal article 2021
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Mooiman, C. (author), Bouwknegt, J. (author), Dekker, W.J.C. (author), Wiersma, S.J. (author), Ortiz Merino, R.A. (author), de Hulster, A.F. (author), Pronk, J.T. (author)All known facultatively fermentative yeasts require molecular oxygen for growth. Only in a small number of yeast species, these requirements can be circumvented by supplementation of known anaerobic growth factors such as nicotinate, sterols and unsaturated fatty acids. Biosynthetic oxygen requirements of yeasts are typically small and,...journal article 2021
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Dekker, W.J.C. (author), Ortiz Merino, R.A. (author), Kaljouw, Astrid (author), Battjes, Julius (author), Wiering, Frank W. (author), Mooiman, C. (author), de la Torre, P. (author), Pronk, J.T. (author)Current large-scale, anaerobic industrial processes for ethanol production from renewable carbohydrates predominantly rely on the mesophilic yeast Saccharomyces cerevisiae. Use of thermotolerant, facultatively fermentative yeasts such as Kluyveromyces marxianus could confer significant economic benefits. However, in contrast to S. cerevisiae,...journal article 2021
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Wiersma, S.J. (author), Mooiman, C. (author), Giera, Martin (author), Pronk, J.T. (author)Biosynthesis of sterols, which are considered essential components of virtually all eukaryotic membranes, requires molecular oxygen. Anaerobic growth of the yeast Saccharomyces cerevisiae therefore strictly depends on sterol supplementation of synthetic growth media. Neocallimastigomycota are a group of strictly anaerobic fungi which, instead...journal article 2020
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Dekker, W.J.C. (author), Wiersma, S.J. (author), Bouwknegt, J. (author), Mooiman, C. (author), Pronk, J.T. (author)In Saccharomyces cerevisiae, acyl-coenzyme A desaturation by Ole1 requires molecular oxygen. Tween 80, a poly-ethoxylated sorbitan-oleate ester, is therefore routinely included in anaerobic growth media as a source of unsaturated fatty acids (UFAs). During optimization of protocols for anaerobic bioreactor cultivation of this yeast, we...journal article 2019
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- Mooiman, D.L. (author) master thesis 2011
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- Mooiman, D.L. (author) report 2010
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- Mooiman, D.L. (author) report 2010
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- Mooiman, D.L. (author) report 2010
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- Mooiman, D.L. (author) report 2010
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Mooiman, M.A. (author), Van den Wollenberg, N. (author)De afdeling Wegenbouw van de provincie Gelderland wil het risicobewustzijn in haar afdeling verankeren en verbreden. Om dit te bewerkstelligen wil de afdeling een methodiek voor risicomanagement implementeren in de bestaande werkprocessen met behulp van ondersteunende tools. Voorafgaand aan dit implementatietraject wil de afdeling voor het einde...report 2008
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Pereboom, D. (author), Mooiman, M. (author), Brouwer, M. (author)De gemeente Reeuwijk kampt met verkeersoverlast door het centrum van de kern. Oplossing voor dit probleem is de aanleg van een rondweg langs de bebouwde kom. De aanleg van deze weg is ingewikkeld, zowel financieel, technisch als maatschappelijk. Om vooraf goed inzicht te krijgen in deze problematiek heeft de gemeente besloten tot het uitvoeren...report 2008
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- Mooiman, T.H.M. (author) master thesis 2008
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- Mooiman, T.H.M. (author) master thesis 2008
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- Mooiman, T.H.M. (author) master thesis 2008
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- Mooiman, J. (author), Goede, E.D. de (author) report 1993
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- Stelling, G.S. (author), Gerritsen, H. (author), Mooiman, J. (author) report 1992