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Sevcenco, A.M. (author), Paravidino, M. (author), Vrouwenvelder, J.S. (author), Wolterbeek, H.T. (author), Van Loosdrecht, M.C.M. (author), Hagen, W.R. (author)
Oxo-anion binding properties of the thermostable enzyme ferritin from Pyrococcus furiosus were characterized with radiography. Radioisotopes 32P and 76As present as oxoanions were used to measure the extent and the rate of their absorption by the ferritin. Thermostable ferritin proved to be an excellent system for rapid phosphate and arsenate...
journal article 2015
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Sevcenco, A.M. (author), Bevers, L.E. (author), Pinkse, M.W.H. (author), Krijger, G.C. (author), Wolterbeek, H.T. (author), Verhaert, P.D.E.M. (author), Hagen, W.R. (author), Hagedoorn, P.L. (author)
The hyperthermophilic archaeon Pyrococcus furiosus expresses five aldehyde oxidoreductase (AOR) enzymes, all containing a tungsto-bispterin cofactor. The growth of this organism is fully dependent on the presence of tungsten in the growth medium. Previous studies have suggested that molybdenum is not incorporated in the active site of these...
journal article 2010
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Sevcenco, A.M. (author), Krijger, G. (author), Pinkse, M. (author), Verhaert, P.D.E.M. (author), Hagen, W.R. (author), Hagedoorn, P.L. (author)
A combination of techniques to separate and quantify the native proteins associated with a particular transition metal ion from a cellular system has been developed. The procedure involves four steps: (1) labeling of the target proteins with a suitable short-lived radioisotope (suitable isotopes are Cu-64, Cu-67, W-187, Mo-99, Zn-69, Mn-56, Ni...
journal article 2009
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Sevcenco, A.M. (author), Pinkse, M. (author), Bol, E. (author), Krijgen, G. (author), Wolterbeek, H. (author), Verhaert, P.D.E.M. (author), Hagedoorn, P.L. (author), Hagen, W.R. (author)
The tungsten metallome of the hyperthermophilic archaeon Pyrococcus furiosus has been investigated using electroanalytical metal analysis and native–native 2D-PAGE with the radioactive tungsten isotope W-187 (t1/2 = 23.9 h). P. furiosus cells have an intracellular tungsten concentration of 29 mM, of which ca. 30% appears to be free tungsten,...
journal article 2009
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