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Olavarria, Karel (author), Pijman, Yared O. (author), Cabrera, Ricardo (author), van Loosdrecht, Mark C.M. (author), Wahl, S.A. (author)The coupling of PHB generation with NADH reoxidation is required to generate PHB as a fermentation product. A fundamental trait to accomplish this feature is to express a functional NADH-preferring acetoacetyl-CoA reductase, engaged in PHB accumulation. One way to obtain such a reductase is by engineering the cofactor preference of the...journal article 2022
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Pei, R. (author), Vicente-Venegas, Gerard (author), van Loosdrecht, Mark C.M. (author), Kleerebezem, R. (author), Werker, A. (author)Polyhydroxyalkanoate accumulation experiments at pilot scale were performed with fullscale municipal waste activated sludge. Development of biomass PHA content was quantified by thermogravimetric analysis. Over 48 h the biomass reached up to 0.49 ± 0.03 gPHA/gVSS (n=4). Samples were processed in parallel to characterise the distribution of...journal article 2022
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Hao, Xiaodi (author), Wang, Xiangyang (author), Shi, Chen (author), van Loosdrecht, Mark C.M. (author), Wu, Yuanyuan (author)Sludge incineration and seawater desalination are two approaches that can be used in the disposal of waste activated sludge (WAS) and for obtaining fresh water. As resource recovery from wastewater treatment and water purification is a topic of particular interest in these times, “water mining” has become a focus of research, with phosphate/P...journal article 2022
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Kouba, Vojtěch (author), Hůrková, Kamila (author), Navrátilová, Klára (author), Kok, Dana (author), Benáková, Andrea (author), Laureni, M. (author), Vodičková, Patricie (author), van Loosdrecht, Mark C.M. (author), Weissbrodt, D.G. (author)The application of partial nitritation-anammox (PN/A) under mainstream conditions can enable substantial cost savings at wastewater treatment plants (WWTPs), but how process conditions and cell physiology affect anammox performance at psychrophilic temperatures below 15 °C remains poorly understood. We tested 14 anammox communities, including...journal article 2022
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Ekholm, Jennifer (author), Persson, Frank (author), de Blois, Mark (author), Modin, Oskar (author), Pronk, M. (author), van Loosdrecht, Mark C.M. (author), Suarez, Carolina (author), Gustavsson, David J.I. (author), Wilén, Britt Marie (author)Aerobic granular sludge (AGS) plants have gained growing interest and application due to their low energy demand, small footprint, and low operational costs. However, the fulfilment of strict discharge limits for nitrogen and phosphorus, vast seasonal temperature variations, and large peaks in influent flows may pose challenges to the...journal article 2022
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Prot, T.J.F. (author), Korving, L. (author), van Loosdrecht, Mark C.M. (author)In a wastewater treatment plant (WWTP), several sludge streams exist and the composition of their liquid phase varies with time and place. For evaluating the potential for formation of precipitates and equilibria for weak acids/bases, the ionic strength and chemical composition need to be known. This information is often not available in...journal article 2022
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Pei, R. (author), Estevez Alonso, A. (author), Ortiz-Seco, Laura (author), van Loosdrecht, Mark C.M. (author), Kleerebezem, R. (author), Werker, A. (author)Municipal activated sludge can be used for polyhydroxyalkanoate (PHA) production, when supplied with volatile fatty acids. In this work, standardized PHA accumulation assays were performed with different activated sludge to determine (1) the maximum biomass PHA content, (2) the degree of enrichment (or volume-to-volume ratio of PHA...journal article 2022
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Goedhart, R.C. (author), Müller, S. (author), van Loosdrecht, Mark C.M. (author), van Halem, D. (author)Iron in anaerobic groundwater is commonly removed by oxidation followed by sand filtration. This produces large volumes of iron(III)(hydr)oxide sludge with little value. Our research investigates the novel concept of anaerobic iron(II) recovery from groundwater as the valuable mineral vivianite (Fe<sub>3</sub>(PO<sub>4</sub>)<sub>2</sub> • 8...journal article 2022
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Prot, T.J.F. (author), Pannekoek, W. (author), Belloni, C. (author), Dugulan, A.I. (author), Hendrikx, R.W.A. (author), Korving, L. (author), van Loosdrecht, Mark C.M. (author)It was recently discovered that vivianite (Fe<sub>3</sub>(PO<sub>4</sub>)<sub>2</sub>.8H<sub>2</sub>O) could be magnetically extracted from digested activated sludge which opened a new route for phosphorus recovery (Wijdeveld et al. 2022). While its formation in digested sludge is regularly reported, it is not yet studied for fresh,...journal article 2022
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Hao, Xiaodi (author), Wu, Daoqi (author), Li, Ji (author), Liu, Ranbin (author), van Loosdrecht, Mark C.M. (author)Entropy is a concept defined by the second law of thermodynamics. Applying this concept to the world we live in, entropy production must be minimized and negentropy (negative entropy production) should be accelerated, in order to produce a healthy and stable ecological system. The present wastewater treatment, however, contributes to entropy...journal article 2022
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Tomas Martinez, S. (author), Chen, L.M. (author), Neu, Thomas R. (author), Weissbrodt, D.G. (author), van Loosdrecht, Mark C.M. (author), Lin, Y. (author)Sialic acids are a family of nine-carbon negatively charged carbohydrates. In animals, they are abundant on mucosa surfaces as terminal carbohydrates of mucin glycoproteins. Some commensal and pathogenic bacteria are able to release, take up and catabolize sialic acids. Recently, sialic acids have been discovered to be widespread among most...journal article 2022
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Kulshreshtha, Y. (author), Vardon, P.J. (author), Meesters, G.M.H. (author), van Loosdrecht, Mark C.M. (author), Mota, Nelson (author), Jonkers, H.M. (author)The water-resistance of cow-dung has made it a widely used stabiliser in traditional earthen structures in several Asian and African countries. Multiple studies have shown an improvement in water-resistance with the addition of cow-dung, but none provides insight into this behaviour. The present study investigates the water-resistance behaviour...journal article 2022
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Calderon Franco, D. (author), Sarelse, R.G. (author), Christou, S. (author), Pronk, M. (author), van Loosdrecht, Mark C.M. (author), Abeel, T.E.P.M.F. (author), Weissbrodt, D.G. (author)In the One Health context, wastewater treatment plants (WWTPs) are central to safeguarding water resources. Nonetheless, many questions remain about their effectiveness in preventing antimicrobial resistance (AMR) dissemination. Most surveillance studies monitor the levels and removal of selected antibiotic resistance genes (ARGs) and mobile...journal article 2022
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van den Berg, L. (author), Toja Ortega, S. (author), van Loosdrecht, Mark C.M. (author), de Kreuk, M.K. (author)Aerobic granular sludge (AGS) is an advanced biofilm-based technology for wastewater treatment. Diffusion of substrates into the granules is a key aspect of this technology. Domestic wastewater contains soluble organic substrates of different sizes that could potentially diffuse into the granules. In this study, the relation between the...journal article 2022
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Elahinik, A. (author), Haarsma, M.N. (author), Abbas, B.A. (author), Pabst, Martin (author), Xevgenos, Dimitris (author), van Loosdrecht, Mark C.M. (author), Pronk, M. (author)Glycerol is abundantly present in wastewater from industries such as biodiesel production facilities. Glycerol is also a potential carbon source for microbes that are involved in wastewater nutrient removal processes. The conversion of glycerol in biological phosphorus removal of aerobic granular sludge processes has not been explored to date....journal article 2022
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Petrik, Leslie Felicia (author), Ngo, Huu Hao (author), Varjani, Sunita (author), Xevgenos, Dimitris (author), Osseweijer, P. (author), van Loosdrecht, Mark C.M. (author), Smol, Marzena (author), Yang, Xuejing (author), Mateo-Sagasta, Javier (author)Eighty percent of wastewater is left untreated or not reused, exacerbating the water quality challenge, especially in vulnerable communities. This Voices asks: how can we improve wastewater management and convert wastewater into a resource?journal article 2022
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Kulshreshtha, Y. (author), Vardon, P.J. (author), Du, Y. (author), Habert, G. (author), Vissac, A. (author), Morel, J.C. (author), van Loosdrecht, Mark C.M. (author), Mota, Nelson (author), Jonkers, H.M. (author)Earthen construction is re-gaining popularity as an ecological and economical alternative to contemporary building materials. While building with earth offers several benefits, its performance due to water ingress is a concern for its widespread application. This limitation is often solved by adding chemical stabilisers such as Portland cement...journal article 2022
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Soler Jofra, A. (author), Schmidtchen, Lisbeth (author), Olmo, Lluc (author), van Loosdrecht, Mark C.M. (author), Pérez, Julio (author)Hydroxylamine is a nitrogen intermediate of ammonium oxidizing bacteria (AOB) that can transiently accumulate during nitrification. The impact of hydroxylamine on aerobic ammonium oxidations is still obscure. In the present study the short and long term impact of hydroxylamine on partial nitritation granular sludge was investigated. Dissolved...journal article 2022
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Wijdeveld, W. K. (author), Prot, T.J.F. (author), Sudintas, G. (author), Kuntke, P. (author), Korving, L. (author), van Loosdrecht, Mark C.M. (author)Phosphorus (P) is an essential resource for food production and chemical industry. Phosphorus use has to become more sustainable and should include phosphorus recycling from secondary sources. About 20% of P ends up in sewage sludge, making this a substantial secondary P source. There is currently a technological gap to recover P from sludge...journal article 2022
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Chen, Xingyu (author), Lee, Yu Jen (author), Yuan, Tian (author), Lei, Zhongfang (author), Adachi, Yasuhisa (author), Zhang, Zhenya (author), Lin, Y. (author), van Loosdrecht, Mark C.M. (author)A reasonable recovery of excess sludge may shift the waste into wealth. Recently an increasing attention has been paid to the recycling of extracellular biopolymers from conventional and advanced biological wastewater treatment systems such as flocculent activated sludge (AS), bacterial aerobic granular sludge (AGS), and algal-bacterial AGS...review 2022